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Series GSE132664 Query DataSets for GSE132664
Status Public on Jul 01, 2019
Title RNAseq of WFU3 mouse prostate epithelial stem cells with knockdown of Map3k7, Chd1, and both
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study is to identify transcriptome-wide changes that occur in a mouse prostate cell line with knockdown of Map3k7(Tak1) and Chd1. We utilize this cell line as a model of an aggressive prostate cancer subtype consisting of loss of both MAP3K7 and CHD1 in human patients. RNAseq comparing shControl, shMap3k7(Tak1), shChd1, and shDouble (both) was used to identify transcriptome changes resulting from loss of these genes of interest in order to identify potential therapeutic vulnerabilites.
Cell line: The generation of the WFU3 cell line has been previously described (Barclay, W.W et al. The Prostate. 2005; PMID:15599944). Breifly, anterior prostate lobes of adult B1/6; 129/SVEV mice were harvested, minced, and extensively digested, and resulting organoids were separated from stromal cells using a Percoll density gradient. These organoids were then plated on rat-tail collagen coated plates in complete growth media, and after 4-5 passages were transferred to uncoated plates. These prostatic epithelial progenitor stem cells are maintained in supplemented 50:50 Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F-12 medium (F12). Complete media components are described in the Barclay, W.W paper.
Overall design mRNA profiles of mouse prostatic epithelial cells (WFU3) with shControl, shMap3k7, shChd1, and both (shMap3k7&shChd1) were generated by RNAsequencing and compared to identify differentially expressed genes between the groups.
Contributor(s) Washino S, Jillson LK, Costello JC, Cramer SD
Citation(s) 31300540
Submission date Jun 13, 2019
Last update date Sep 16, 2019
Contact name Lauren Jillson
Organization name University of Colorado Anschutz Medical Campus
Department Pharmacology
Lab Scott Cramer
Street address 12801 E 17th Avenue, Room L18-6213, Mail stop 8303
City Aurora
State/province Colorado
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (11)
GSM3885202 Wctrlpn
GSM3885203 Wctrl4
GSM3885204 Wctrl5
BioProject PRJNA548679
SRA SRP201304

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Supplementary file Size Download File type/resource
GSE132664_WFU3_compiled_normalized.txt.gz 1001.7 Kb (ftp)(http) TXT
GSE132664_WFU3_compiled_raw.txt.gz 614.8 Kb (ftp)(http) TXT
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