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| Status |
Public on Jul 01, 2019 |
| Title |
RNAseq of WFU3 mouse prostate epithelial stem cells with knockdown of Map3k7, Chd1, and both |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to identify transcriptome-wide changes that occur in a mouse prostate cell line with knockdown of Map3k7(Tak1) and Chd1. We utilize this cell line as a model of an aggressive prostate cancer subtype consisting of loss of both MAP3K7 and CHD1 in human patients. RNAseq comparing shControl, shMap3k7(Tak1), shChd1, and shDouble (both) was used to identify transcriptome changes resulting from loss of these genes of interest in order to identify potential therapeutic vulnerabilites.
Cell line: The generation of the WFU3 cell line has been previously described (Barclay, W.W et al. The Prostate. 2005; PMID:15599944). Breifly, anterior prostate lobes of adult B1/6; 129/SVEV mice were harvested, minced, and extensively digested, and resulting organoids were separated from stromal cells using a Percoll density gradient. These organoids were then plated on rat-tail collagen coated plates in complete growth media, and after 4-5 passages were transferred to uncoated plates. These prostatic epithelial progenitor stem cells are maintained in supplemented 50:50 Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F-12 medium (F12). Complete media components are described in the Barclay, W.W paper.
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| Overall design |
mRNA profiles of mouse prostatic epithelial cells (WFU3) with shControl, shMap3k7, shChd1, and both (shMap3k7&shChd1) were generated by RNAsequencing and compared to identify differentially expressed genes between the groups.
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| Contributor(s) |
Washino S, Jillson LK, Costello JC, Cramer SD |
| Citation(s) |
31300540 |
| Submission date |
Jun 13, 2019 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Lauren Jillson |
| E-mail(s) |
lauren.jillson@ucdenver.edu
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| Organization name |
University of Colorado Anschutz Medical Campus
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| Department |
Pharmacology
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| Lab |
Scott Cramer
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| Street address |
12801 E 17th Avenue, Room L18-6213, Mail stop 8303
|
| City |
Aurora |
| State/province |
Colorado |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA548679 |
| SRA |
SRP201304 |
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