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Series GSE132591 Query DataSets for GSE132591
Status Public on Jun 04, 2020
Title RNA-seq analysis of mESCs cultured on either Laminin-511 or gelatin coated dishes in either Std or 2i medium
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Some mouse embryonic stem cell (mESC) lines need to be maintained on feeder cells in gelatin/Std condition. To eliminate the need for feeder cells, we decided to maintain the C57BL6/J mESCs on dishes coated with Laminin-511 (LN511) enabling maintenance of mESCs without feeder cells in Std condition (Domogatskaya et al., 2008). To compare the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on Laminin-511 coated dish, we performed RNA-Seq analysis. We found that the transcriptomes of mESCs cultured on gelatin-coated dish and those cultured on LN511 coated dish showed no considerable difference in expression patterns.
Overall design Examination of 2 coating conditions in 2 medium. There are two biological replication in each condition.
Contributor(s) Ochiai H, Nikaido I
Citation(s) 32596448
Submission date Jun 12, 2019
Last update date Jul 15, 2020
Contact name Itoshi NIKAIDO
Organization name RIKEN
Department Center for Biosystems Dynamics Research
Lab Laboratory for Bioinformatics Research
Street address 2-1 Hirosawa
City Wako
State/province Saitama
ZIP/Postal code 351-0198
Country Japan
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM3878570 LM511_2i_1
GSM3878571 LM511_2i_2
GSM3878572 LM511_std_1
This SubSeries is part of SuperSeries:
GSE132593 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells
BioProject PRJNA548638
SRA SRP201376

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132591_genes.fpkm.csv.gz 372.3 Kb (ftp)(http) CSV
GSE132591_isoforms.fpkm.csv.gz 989.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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