GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE132590 Query DataSets for GSE132590
Status Public on Jun 04, 2020
Title Genome-wide CRISPR library screening to identify genes involved in the regulation of the kinetic properties of transcriptional bursting
Organism Mus musculus
Experiment type Other
Summary Cell-to-cell heterogeneity in gene expression can even be observed in the same type of cells, present in a similar environment. Transcriptional bursting is thought to be one of contributing factors to the heterogeneity, but it remains elusive how the kinetic properties of transcriptional bursting (e.g. burst size, burst frequency, and noise induced by transcriptional bursting) are regulated in mammalian cells. To unbiasedly identify genes regulating the kinetic properties of transcriptional bursting, we performed large-scale CRISPR/-Cas9 based screening and functional analysis, and found that Akt/MAPK signaling pathways are involved in the regulation of the kinetic properties of transcriptional bursting.
Overall design Genome-wide CRISPR library screening was performed in Nanog, Dnmt3l, Trim28 knockin cell lines, in which GFP and iRFP reporter separately inserted into the two alleles. There are two technical replication in FACS-sorted sampels.
Contributor(s) Ochiai H, Nikaido I
Citation(s) 32596448
Submission date Jun 12, 2019
Last update date Jul 15, 2020
Contact name Itoshi NIKAIDO
Organization name RIKEN
Department Center for Biosystems Dynamics Research
Lab Laboratory for Bioinformatics Research
Street address 2-1 Hirosawa
City Wako
State/province Saitama
ZIP/Postal code 351-0198
Country Japan
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM3878560 GeCKO-Nanog_UNSORT
GSM3878561 GeCKO-Nanog_SORT_T1
GSM3878562 GeCKO-Nanog_SORT_T2
This SubSeries is part of SuperSeries:
GSE132593 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells
BioProject PRJNA548636
SRA SRP201375

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132590_FASTA_GeckoV2_Mouse_A.csv.gz 806.0 Kb (ftp)(http) CSV
GSE132590_FASTA_GeckoV2_Mouse_A_controlList.txt.gz 7.5 Kb (ftp)(http) TXT
GSE132590_gecko_low.count.txt.gz 1.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap