|
| Status |
Public on Aug 08, 2019 |
| Title |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [mRNA_TC] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Extensive analysis if the means by which ERK affects transcription via enhancer regulation in mouse embryonic stem cells. This study combines analysis of mRNA, and nascent RNA dynamics and correlates this with changes in transcription factor, co-factor, and RNAPII binding and activity.
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| |
|
| Overall design |
Mouse ESCs expressing a cRAF-ERT2 construct allowing ERK activation upon the addition of 4OT to the media. Cells were left untreated (0h), or treated with 250nM 4OHT for 1-2-or 4hrs. Total RNA was collected and purified and rRNA contamination was subsequently removed using the ribogold kit from Illumina prior to library preparation.
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| |
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| Contributor(s) |
Brickman JM, Hamilton WB |
| Citation(s) |
31695196 |
| |
| Submission date |
Jun 10, 2019 |
| Last update date |
Nov 11, 2019 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
|
| GSM3869979 |
Clone a, 4OHT treated for 1hr [mRNA_TC] |
| GSM3869980 |
Clone b, 4OHT treated for 1hr [mRNA_TC] |
| GSM3869981 |
Clone c, 4OHT treated for 1hr [mRNA_TC] |
| GSM3869982 |
Clone a, 4OHT treated for 2hrs [mRNA_TC] |
| GSM3869983 |
Clone b, 4OHT treated for 2hrs [mRNA_TC] |
| GSM3869984 |
Clone c, 4OHT treated for 2hrs [mRNA_TC] |
| GSM3869985 |
Clone a, 4OHT treated for 4hrs [mRNA_TC] |
| GSM3869986 |
Clone b, 4OHT treated for 4hrs [mRNA_TC] |
| GSM3869987 |
Clone c, 4OHT treated for 4hrs [mRNA_TC] |
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| This SubSeries is part of SuperSeries: |
| GSE132444 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation |
|
| Relations |
| BioProject |
PRJNA548184 |
| SRA |
SRP201033 |
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