|
| Status |
Public on Aug 08, 2019 |
| Title |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [H3K27me3] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Extensive analysis if the means by which ERK affects transcription via enhancer regulation in mouse embryonic stem cells. This study combines analysis of mRNA, and nascent RNA dynamics and correlates this with changes in transcription factor, co-factor, and RNAPII binding and activity.
|
| |
|
| Overall design |
Mouse embryonic stem cells, stably expressing a cRAF-ErT2 expression cassette, were treated with 4OHT for 2hrs to activate ERK signalling via cRAF-ErT2
|
| |
|
| Contributor(s) |
Brickman JM, Hamilton WB |
| Citation(s) |
31695196 |
| |
| Submission date |
Jun 10, 2019 |
| Last update date |
Nov 11, 2019 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (8)
|
| GSM3869924 |
Input Clone a, untreated [H3K27me3] |
| GSM3869925 |
Input Clone b, untreated [H3K27me3] |
| GSM3869926 |
Input Clone a, 4OHT treated for 2hrs [H3K27me3] |
| GSM3869927 |
Input Clone b, 4OHT treated for 2hrs [H3K27me3] |
| GSM3869928 |
IP Clone a, untreated [H3K27me3] |
| GSM3869929 |
IP Clone b, untreated [H3K27me3] |
| GSM3869930 |
IP Clone a, 4OHT treated for 2hrs [H3K27me3] |
| GSM3869931 |
IP Clone b, 4OHT treated for 2hrs [H3K27me3] |
|
| This SubSeries is part of SuperSeries: |
| GSE132444 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation |
|
| Relations |
| BioProject |
PRJNA548179 |
| SRA |
SRP200998 |
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