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Status |
Public on Jun 10, 2020 |
Title |
Expression profiling by RNA-Seq of adipose tissue in mice after partial nephrectomy and administration of pNaKtide |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We performed expression profiling by RNA-Seq on C57BL6 mice at 10 weeks of age. Mice were divided into four groups and either underwent partial nephrectomy (PNx) or sham (CTR) operation, and were adminstered either pNaKtide (NaK) or GFP.
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Overall design |
RNA was extracted from adipose tissue from six 10-week old mice in each group; PNx-GFP, CTR-GFP, PNx-GFP, or PNx-NaK, and sequenced in a 2x50 base-pair design on an Illumina 1500 sequencer.
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Contributor(s) |
Sodhi K, Chaudhary MA, Wang X, Lakhani HV, Nawab A, Zehra M, Pratt R, Cottrill CL, Snoad B, Fan J, Denvir J, Liu J, Xie Z, Abraham NG, Shapiro JI |
Citation(s) |
32824688 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
P20 GM103434 |
Bioinformatics Core |
MARSHALL UNIVERSITY RESEARCH CORPORATION |
MARY E DAVIS |
P20 GM103434 |
Genomics Core |
MARSHALL UNIVERSITY RESEARCH CORPORATION |
Donald Anthony Primerano |
P20 GM103434 |
West Virginia IDeA Network of Biomedical Research Excellence (WV-INBRE) |
MARSHALL UNIVERSITY RESEARCH CORPORATION |
GARY O. RANKIN |
P20 GM121299 |
Appalachian Center for Cellular transport in Obesity Related Disorders (ACCORD) |
MARSHALL UNIVERSITY RESEARCH CORPORATION |
Uma Sundaram |
U54 GM104942 |
West Virginia Clinical and Translational Science Institute: Improving Health through Partnerships and Transformative Research |
WEST VIRGINIA UNIVERSITY RESEARCH CORPORATION |
Sally Lynn Hodder |
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Submission date |
Jun 10, 2019 |
Last update date |
Sep 08, 2020 |
Contact name |
James Denvir |
E-mail(s) |
denvir@marshall.edu
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Organization name |
Marshall University School of Medicine
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Department |
Biochemistry and Microbiology
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Lab |
Genomics and Bioinformatics Core Facility
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Street address |
One John Marshall Drive
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City |
Huntington |
State/province |
WV |
ZIP/Postal code |
25755 |
Country |
USA |
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Platforms (1) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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Samples (24)
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This SubSeries is part of SuperSeries: |
GSE152725 |
Oxidant Alterations in Adipocyte mRNA Expression: Role of the Na,K-ATPase Oxidant Amplification Loop |
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Relations |
BioProject |
PRJNA548122 |
SRA |
SRP200975 |