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Series GSE132420 Query DataSets for GSE132420
Status Public on Jun 07, 2022
Title Normal and Sjogren’s syndrome models of the murine lacrimal gland studied at single-cell resolution
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The lacrimal gland is of central interest in ophthalmology both as the source of the aqueous component of tear fluid and as the site of autoimmune pathology in the context of Sjogren’s syndrome (SjS). To provide a foundational description of mouse lacrimal gland cell types and their patterns of gene expression, we have analyzed single-cell transcriptomes from wild-type (Balb/c) mice and from two genetically based SjS models, MRL/lpr and NOD (nonobese diabetic).H2b, and defined the localization of multiple cell-type-specific protein and mRNA markers. This analysis has uncovered a previously undescribed cell type, Car6+ cells, which are located at the junction of the acini and the connecting ducts. More than a dozen secreted polypeptides that are likely to be components of tear fluid are expressed by acinar cells and show pronounced sex differences in expression. Additional examples of gene expression heterogeneity within a single cell type were identified, including a gradient of Claudin4 along the length of the ductal system and cell-to-cell heterogeneity in transcription factor expression within acinar and myoepithelial cells. The patterns of expression of channels, transporters, and pumps in acinar, Car6+, and ductal cells make strong predictions regarding the mechanisms of water and electrolyte secretion. In MRL/lpr and NOD.H2b lacrimal glands, distinctive changes in parenchymal gene expression and in immune cell subsets reveal widespread interferon responses, a T cell–dominated infiltrate in the MRL/lpr model, and a mixed B cell and T cell infiltrate in the NOD.H2b model.
Overall design Single-cell RNAseq of BALB/cJ, MRL/MpJ-Fas(lpr/lpr), NOD.H2b lacrimal glands.

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Contributor(s) Heng JS, Rattner A, Winer BL, Goff LA, Nathans J
Citation(s) 37812717
Submission date Jun 09, 2019
Last update date Oct 16, 2023
Contact name Jacob S Heng
Organization name Johns Hopkins University School of Medicine
Department Molecular Biology and Genetics
Lab Nathans Lab
Street address 725 North Wolfe Street
City Baltimore
State/province MD
ZIP/Postal code 21210
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM3863114 JH43_WT_female_lacrimal_scRNAseq_R1
GSM3863115 JH44_MRL_lpr_female_lacrimal_scRNAseq_R1
GSM3863116 JH45_WT_female_lacrimal_scRNAseq_R2
BioProject PRJNA548013
SRA SRP200952

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Supplementary file Size Download File type/resource
GSE132420_RAW.tar 202.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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