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Series GSE132154 Query DataSets for GSE132154
Status Public on Oct 01, 2019
Title Dual modulation of neuron specific microRNAs and the REST complex promotes functional maturation of induced human adult neurons
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Direct neural reprogramming can be achieved using different approaches, including by expressing neuronal transcription factors or microRNAs, and by knocking down neuronal repressive elements. However, there still exists a high variability in terms of the quality and maturity of the induced neurons (iNs) obtained, depending on the reprogramming strategy employed. Here, we evaluate different long-term culture conditions and study the effect of expressing the neuronal-specific microRNAs miR124 and miR9/9* while reprogramming with Ascl1, Brn2 and knockdown of the neuronal repressor REST. We show that addition of microRNA supports neuronal maturation both in terms of gene and protein expression, as well as promotes the development of active ion channels and the ability of iNs to generate current induced and spontaneous action potentials.
Overall design RNA sequencing of induced human neurons from fibroblasts with two different conditions. Three replicatess were used for each condition.
Contributor(s) Sharma Y
Citation(s) 31535361
Submission date Jun 04, 2019
Last update date Jan 09, 2020
Contact name Yogita Sharma
Organization name Lund University
Street address Solvegatan 17
City Lund
ZIP/Postal code 22184
Country Sweden
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3851668 C2_REST_sh-PBmPA-Day24_1
GSM3851669 C2_REST_sh-PBmPA-Day24_2
GSM3851670 C2_REST_sh-PBmPA-Day24_3
BioProject PRJNA546243
SRA SRP200390

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Supplementary file Size Download File type/resource
GSE132154_Table_1A_rawcounts_Day5.tsv.gz 293.0 Kb (ftp)(http) TSV
GSE132154_Table_1B_rawcounts_Day24.tsv.gz 385.1 Kb (ftp)(http) TSV
GSE132154_Table_2A_d5.NormalisedCounts.tsv.gz 702.4 Kb (ftp)(http) TSV
GSE132154_Table_2B_d24.NormalisedCounts.tsv.gz 698.7 Kb (ftp)(http) TSV
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Processed data are available on Series record

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