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Series GSE132045 Query DataSets for GSE132045
Status Public on Sep 24, 2019
Title Single cell RNA-seq Analysis of Wild Type(WT), Dux knockout(KO) and Dux overexpression(OE) embryos
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We tested the transcriptome of embryos from WT and Dux KO mice including zygotes (hCG 28h), early 2-cell (hCG 31-32h), middle 2-cell(hCG 41-42h), late 2-cell(hCG 47-48h) and, Dux mRNA injected embryos including zygote(5h after injection, hCG 28h), early 4-cell(6h after injection, hCG 54h) and late 4-cell (17h after injection, hCG 65h) using the Covaris DNA shearing protocol for Smart-seq sequence library generation. We found that the activation of Dux is important but not essential for ZGA, but the silencing and elimination of Dux is strictly necessary for early embryonic development.
Overall design Embryos mRNA profiles of WT,Dux KO and Dux OE were generated by deep sequencing, using Illumina NovaSeq 6000.
Contributor(s) Guo M, Zhang Y, Wang Y
Citation(s) 31591446
Submission date May 31, 2019
Last update date Oct 30, 2019
Contact name Mingyue Guo
Phone 86-21-65982278
Organization name Tongji University
Department School of Life science and technology
Lab Gao Shaorong
Street address Siping
City Shanghai
State/province Shanghai
ZIP/Postal code 200092
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (99)
GSM3877998 Ctrl.4cellEarly.rep1
GSM3877999 Ctrl.4cellEarly.rep10
GSM3878000 Ctrl.4cellEarly.rep2
This SubSeries is part of SuperSeries:
GSE134832 Precise temporal regulation of Dux is important for embryo development
BioProject PRJNA548396
SRA SRP201141

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132045_RAW.tar 45.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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