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Series GSE131941 Query DataSets for GSE131941
Status Public on Sep 29, 2020
Title Myc is required for adaptive beta cell replication in young mice but is not sufficient in one year old mice fed with a high fat
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Purpose: To test the role of Myc in adaptive expansion of beta cells
Methods: Islets were isolated from 8 week old or one year old male C57BL6 mice fed a standard or high fat diet. RNAseq and DNA mehtylseq was performed.
Results: RNAseq showed that Myc target genes are upregulted in young, but not old mice after a short term high fat diet. DNA methylation analysis shows hypomethylation of cell cycle genes that are Myc targets in islets from young mice fed with a HFD. Importantly, DNA hypomethylation of Myc response elements does not occur in islets from one-year-old mice fed short-term HFD
Conclusions: We conclude that Myc is required for metabolic stress-mediated beta cell expansion in young mice, but with aging Myc upregulation in beta cells is not sufficient to induce beta cell replication by, at least partly, an epigenetically-mediated resistance to Myc action.
Overall design RNASeq and MethylSeq of selected genomic regions from islets isolated from 8 week old or 1 year old mice fed a standard or high fat diet. All groups in triplicate or quadruplicate.
Contributor(s) Garcia-Ocana A, Scott DK
Citation(s) 31292135
Submission date May 29, 2019
Last update date Sep 29, 2020
Contact name Donald K Scott
Phone 4129926061
Organization name MSSM
Department Diabetes Obesity and Metabolism Institute
Lab Scott
Street address 1428 Madison Avenue
City New York
State/province New York
ZIP/Postal code 10029
Country USA
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (29)
GSM3831301 Young RD 1 RNAseq
GSM3831302 Young RD 2 RNAseq
GSM3831303 Young RD 3 RNAseq
BioProject PRJNA545355
SRA SRP199803

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131941_DESeq2_results_HFD_vs_SD_old.txt.gz 2.6 Mb (ftp)(http) TXT
GSE131941_DESeq2_results_HFD_vs_SD_young.txt.gz 2.6 Mb (ftp)(http) TXT
GSE131941_QC_statistics_to_deliver_old.txt.gz 631 b (ftp)(http) TXT
GSE131941_QC_statistics_to_deliver_young.txt.gz 530 b (ftp)(http) TXT
GSE131941_RAW.tar 44.2 Mb (http)(custom) TAR (of TXT)
GSE131941_featureCounts_count_matrix_old.txt.gz 476.0 Kb (ftp)(http) TXT
GSE131941_featureCounts_count_matrix_young.txt.gz 397.0 Kb (ftp)(http) TXT
GSE131941_kallisto_est_count_matrix_old.txt.gz 2.2 Mb (ftp)(http) TXT
GSE131941_kallisto_est_count_matrix_young.txt.gz 1.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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