|
| Status |
Public on Sep 29, 2020 |
| Title |
Myc is required for adaptive beta cell replication in young mice but is not sufficient in one year old mice fed with a high fat |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
Purpose: To test the role of Myc in adaptive expansion of beta cells
Methods: Islets were isolated from 8 week old or one year old male C57BL6 mice fed a standard or high fat diet. RNAseq and DNA mehtylseq was performed.
Results: RNAseq showed that Myc target genes are upregulted in young, but not old mice after a short term high fat diet. DNA methylation analysis shows hypomethylation of cell cycle genes that are Myc targets in islets from young mice fed with a HFD. Importantly, DNA hypomethylation of Myc response elements does not occur in islets from one-year-old mice fed short-term HFD
Conclusions: We conclude that Myc is required for metabolic stress-mediated beta cell expansion in young mice, but with aging Myc upregulation in beta cells is not sufficient to induce beta cell replication by, at least partly, an epigenetically-mediated resistance to Myc action.
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| Overall design |
RNASeq and MethylSeq of selected genomic regions from islets isolated from 8 week old or 1 year old mice fed a standard or high fat diet. All groups in triplicate or quadruplicate.
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| |
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| Contributor(s) |
Garcia-Ocana A, Scott DK |
| Citation(s) |
31292135 |
| |
| Submission date |
May 29, 2019 |
| Last update date |
Sep 29, 2020 |
| Contact name |
Donald K Scott |
| E-mail(s) |
donald.scott@mssm.edu
|
| Phone |
4129926061
|
| Organization name |
MSSM
|
| Department |
Diabetes Obesity and Metabolism Institute
|
| Lab |
Scott
|
| Street address |
1428 Madison Avenue
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (29)
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| Relations |
| BioProject |
PRJNA545355 |
| SRA |
SRP199803 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131941_DESeq2_results_HFD_vs_SD_old.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE131941_DESeq2_results_HFD_vs_SD_young.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE131941_QC_statistics_to_deliver_old.txt.gz |
631 b |
(ftp)(http) |
TXT |
| GSE131941_QC_statistics_to_deliver_young.txt.gz |
530 b |
(ftp)(http) |
TXT |
| GSE131941_RAW.tar |
44.2 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE131941_featureCounts_count_matrix_old.txt.gz |
476.0 Kb |
(ftp)(http) |
TXT |
| GSE131941_featureCounts_count_matrix_young.txt.gz |
397.0 Kb |
(ftp)(http) |
TXT |
| GSE131941_kallisto_est_count_matrix_old.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
| GSE131941_kallisto_est_count_matrix_young.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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