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Series GSE131933 Query DataSets for GSE131933
Status Public on Sep 01, 2021
Title Effects of durvalumab alone or combined with tremelimumab on preexisting cells in the tumor microenvironment
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Immune checkpoint blockade with anti–PD-1/L1 or anti–CTLA-4 therapies has shown promising results across multiple cancer types, but the mechanisms within the tumor microenvironment (TME) have not been fully characterized. We used single-cell RNA sequencing to investigate the effects of anti–PD-L1 monoclonal antibody (mAb) durvalumab (D) alone and combined with an anti–CTLA-4 mAb, tremelimumab (T), on distinct subsets of immune cells. Upon treatment with D or D+T, two NSCLC tumors showed elevated IFNγ responses, although they differed in magnitude of response but differed in other areas. Our results highlight that D and D+T in the TME have some consistent effects across tumors but also exhibit tumor-specific effects depending on composition and functional state of immune cells in the TME at time of treatment. This complex tumor-specific interplay of diverse immune subtypes in the TME is critical to better understand patient response to immunotherapy.
Overall design Two tumors were treated by Durva and Durva+Treme and underwent single cell sequencing. RNA sequencing of 97 advanced-stage non–small-cell lung carcinoma (NSCLC) biopsies from a nonrandomized phase 1b/2 clinical trial (1108/NCT01693562) were profiled to identify expression of genes of interest.
Contributor(s) Shrestha Y
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Submission date May 29, 2019
Last update date Sep 01, 2021
Contact name Yinong Sebastian
Organization name Medimmune
Street address One MedImmune Way
City Gaithersburg
ZIP/Postal code 20878
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM3830056 T1_D0
GSM3830057 T1_D7_IsoCtrl
GSM3830058 T1_D7_Durva
BioProject PRJNA545340
SRA SRP199793

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Supplementary file Size Download File type/resource
GSE131933_T1D7_gene_count.txt.gz 914.2 Mb (ftp)(http) TXT
GSE131933_T1T3D0_gene_count.txt.gz 25.5 Mb (ftp)(http) TXT
GSE131933_T3D11_gene_count.txt.gz 86.4 Mb (ftp)(http) TXT
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Processed data are available on Series record

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