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Series GSE131932 Query DataSets for GSE131932
Status Public on Aug 08, 2019
Title Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [RBP1 ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Extensive analysis if the means by which ERK affects transcription via enhancer regulation in mouse embryonic stem cells. This study combines analysis of mRNA, and nascent RNA dynamics and correlates this with changes in transcription factor, co-factor, and RNAPII binding and activity.
Overall design Mouse embryonic stem cells, stably expressing a cRAF-ErT2 expression cassette, were treated with 4OHT to activate ERK signalling via cRAF-ErT2 for either 2hrs, 8hrs, or 8hrs followed by a subsequent 2hr treatement with the MEK inhibitor PD0325901.
Contributor(s) Brickman JM, Hamilton WB
Citation(s) 31695196
Submission date May 29, 2019
Last update date Nov 11, 2019
Contact name Josh M Brickman
Organization name Copenhagen University
Department reNEW
Lab Brickman
Street address 3B Blegdamsvej
City Copenhagen
ZIP/Postal code 2200
Country Denmark
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (22)
GSM3830034 0h clone a input for RBP1 ChIP
GSM3830035 0h clone b input for RBP1 ChIP
GSM3830036 0h clone c input for RBP1 ChIP
This SubSeries is part of SuperSeries:
GSE132444 Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation
BioProject PRJNA545337
SRA SRP199787

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131932_supplemental_table_8.xlsx 2.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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