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Series GSE131723 Query DataSets for GSE131723
Status Public on Oct 28, 2020
Title Bisulfite-free epigenomics and genomics of single cells analyzed by methylation sensitive restriction (epi-gSCAR)
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between two cell lines based on the DNA methylation and genetic profiles.
Overall design DNA methylation and gene variant analysis of 27 single Kasumi-1 and 20 single OCI-AML3 AML cells
Contributor(s) Niemöller C, Wehrle J, Becker H
Citation(s) 33526904
Submission date May 23, 2019
Last update date Feb 03, 2021
Contact name Heiko Becker
Organization name University Medical Center
Department Department of Medicine I
Lab Becker
Street address Breisacher Straße 115
City Freiburg
State/province Baden-Württemberg
ZIP/Postal code 79106
Country Germany
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (49)
GSM3814736 Kasumi-1_single-cell_1
GSM3814737 Kasumi-1_single-cell_2
GSM3814738 Kasumi-1_single-cell_3
BioProject PRJNA544519
SRA SRP199364

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Supplementary file Size Download File type/resource
GSE131723_RAW.tar 105.7 Mb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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