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Series GSE1317 Query DataSets for GSE1317
Status Public on Apr 17, 2004
Title Ultraviolet induced gene expression in human basal cell culture
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This data set was generated as part of Biogen Community Lab's science fair mentorship program. In brief, human basal cells (ATCC: CRL-7761, designation TE 353.Sk) were cultured to 80% confluence. After removing media, cells were treated with a titration of ultraviolet light (Biorad GS GeneLinker, 254 nm energy maximum). Media was then added and cells were grown for 24 hours. Total RNA was harvested using RLT buffer and Qiagen RNeasy kit. 5 ug of total RNA was labeled using standard Affymetrix protocols for Eukaryotic target preparation (GeneChip Expression Analysis Technical Manual Revision 4). GeneChip intensity values were scaled to 2500 RFU.
Keywords = UV
Keywords = ultraviolet
Keywords = basal cell
Keywords: dose response
Contributor(s) Zou X, Shearstone JR
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Submission date Apr 15, 2004
Last update date Aug 10, 2018
Contact name Jeff Shearstone
Phone 617-245-1321
Organization name Acetylon Pharmaceuticals
Department Biology
Street address 70 Fargo Street
City Boston
State/province MA
ZIP/Postal code 02210
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (3)
GSM21706 Normal basal cell - 0mJ
GSM21707 Normal basal cell - 20mJ
GSM21708 Normal basal cell - 40mJ
BioProject PRJNA89885

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Supplementary file Size Download File type/resource
GSE1317_RAW.tar 126.4 Mb (http)(custom) TAR (of CEL, DAT)

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