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Series GSE131696 Query DataSets for GSE131696
Status Public on Aug 20, 2020
Title Sexually dimorphic crosstalk at the maternal-fetal interface
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The first trimester is a critical window of maternal-fetal communication for pregnancy. Using single cell RNA-sequencing to dissect placenta heterogeneity, we identified five major cell types (trophoblasts, stromal cells, hofbauer cells, antigen presenting cells and endothelial cells). We identified seven unique trophoblast subclusters, including new subtypes that transition into the terminal cell types, extra-villous trophoblasts and syncytiotrophoblasts. As fetal sex impacts pregnancy, we analyzed sex differences in each cell type and identified differences in immune cell function. TGFβ1, β-estradiol, and dihydrotestosterone emerge as upstream regulators of sexually dimorphic genes in a cell type specific manner. Thus, the fetal contribution at the maternal-fetal interface is cell and sex specific.
Overall design Examination of transcriptomic profiles at the single cell level in the primary tissues from first trimester human placenta. In total, 6 placental tissues were utilized, of which 3 were from female conceptions and 3 were from male conceptions.
Contributor(s) Pisarska MD, Sun T, Gonzalez TL, Deng N, DiPentino R, Clark EL, Lee B, Tang J, Wang Y, Stripp BR, Yao C, Tseng H, Karumanchi SA, Koeppel AF, Turner SD, Farber CR, Rich SS, Wang ET, Williams III J
Citation(s) 32772088
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HD074368 Adverse Outcomes of Assisted Reproductive Technologies: Genetics or Epigenetics? CEDARS-SINAI MEDICAL CENTER Margareta Pisarska
R01 HD091773 Noncoding RNA regulation of the human placental transcriptome among the sexes CEDARS-SINAI MEDICAL CENTER Margareta Pisarska
U01 EB026421 Non-Invasive Prenatal Diagnostics Based on Circulating Trophoblasts UNIVERSITY OF CALIFORNIA LOS ANGELES Margareta Pisarska
T32 DK007770 Training Program in Endocrinology, Diabetes, and Metabolism CEDARS-SINAI MEDICAL CENTER SHLOMO MELMED
Submission date May 23, 2019
Last update date Aug 20, 2020
Contact name Margareta D Pisarska
Phone 3106148307
Organization name Cedars Sinai Medical Center
Department OB/GYN, Div REI
Lab Pisarska
Street address 8700 Beverly Blvd
City Los Angeles
State/province CA
ZIP/Postal code 90048
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM3814116 72406
GSM3814117 72424
GSM3814118 72426
This SubSeries is part of SuperSeries:
GSE131875 Sexually dimorphic crosstalk at the maternal-fetal interface
BioProject PRJNA544522
SRA SRP199367

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131696_6sample_Expr_raw.csv.gz 20.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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