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Series GSE131507 Query DataSets for GSE131507
Status Public on Jun 01, 2020
Title Gene regulation by restoration of KMT2C in human colorectal cancer cells HCT116 and RKO
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The histone 3 lysine 4 (H3K4) monomethylase KMT2C is mutated across several cancer types, however the effects of mutations on epigenome organization, gene expression and cell growth are not clear. To study effects of KMT2C expression in CRC cells we restored one allele to wild type KMT2C in the two CRC cell lines RKO and HCT116, which both are homozygous KMT2C c.8390delA mutant, by gene editing. RNA sequencing was performed to profile gene expression when KMT2C was restored in RKO and HCT116 cells.
Overall design Three independently isolated RNA samples each were sequenced from parental cell lines HCT116 and RKO. Single samples were sequenced for KMT2C knock-in clones, except RKO KMT2C KI2 where duplicate samples were sequenced. Gene expression levels in KMT2C knock-in clones were compared to those in the respective parental cells.
Contributor(s) Larsson C, Sjöblom T
Citation(s) 32471474
Submission date May 20, 2019
Last update date Jun 03, 2020
Contact name L. H.
Organization name Uppsala University
Street address Dag Hammarskjölds Väg 20
City Uppsala
ZIP/Postal code 751 85
Country Sweden
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (12)
GSM3782962 HCT116_1
GSM3782963 HCT116_2
GSM3782964 HCT116_3
BioProject PRJNA543871
SRA SRP198978

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131507_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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