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Series GSE131502 Query DataSets for GSE131502
Status Public on Apr 24, 2020
Title Proline Glutamic acid and Leucine rich Protein 1 (PELP1) promotes glioblastoma progression by enhancing Wnt/β-catenin signaling
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We examined the transcriptional changes modulated by Proline Glutamic acid and Leucine rich Protein 1 (PELP1) by performing global transcriptome analysis. U87 cells were transfected with control siRNA or PELP1 siRNA and the RNA was isolated and utilized for RNA-seq analysis. Our results demonstrated that PELP1 modulated genes were related to inhibition of matrix metalloproteases, glioma invasion signaling, Wnt/β-catenin signaling and other pathways related to invasion and GBM signaling.
 
Overall design Total RNA was isolated from U87 cells transfected with control siRNA or PELP1 siRNA. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 2000 in duplicate. The combined raw reads were aligned to UCSC hg19 and genes were annotated by Tophat. Genes were annotated and quantified by HTSeq-DESeq pipeline.
 
Contributor(s) Sareddy GR, Chen Y, Vadlamudi RK
Citation(s) 32309805
Submission date May 20, 2019
Last update date Apr 24, 2020
Contact name YI ZOU
E-mail(s) zou@uthscsa.edu
Organization name UTHSA-GCCRI
Street address 8403 Floyd Curl Drive
City SAN ANTONIO
State/province TX
ZIP/Postal code 78229
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM3782926 U87_ control siRNA_1
GSM3782927 U87_ control siRNA_2
GSM3782928 U87_ PELP1 siRNA_1
Relations
BioProject PRJNA543856
SRA SRP198956

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131502_RAW.tar 380.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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