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Series GSE131316 Query DataSets for GSE131316
Status Public on Apr 21, 2020
Title m6A-Label-seq directly reports m6A methylome at base resolution
Organisms Homo sapiens; Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Here we report a metabolic labeling method to map mRNA N6-methyladenosine (m6A) modification transcriptome-wide at base resolution, termed m6A-label-seq. The cells were fed with Se-allyl-L-selenohomocysteine, an analog of methoine, which serves as the precursor of methylation enzyme cofactor, so that cellular RNAs were continuously deposited with N6-allyladenosine (a6A) at supposed m6A sites. We enriched a6A-containing mRNAs and sequenced their a6A sites which are identical to m6A sites, based on iodination-induced misincorporation during reverse transcription.
Overall design We profiled m6A sites at base resolution in human HeLa and HEK293T cells and mouse H2.35 cells by metabolically incorporation of Se-derived methionine analog.
We assessed the potential stress response in HeLa cells by an RNA-seq experiment.
Contributor(s) Cao J
Citation(s) 32341503
Submission date May 16, 2019
Last update date Jul 22, 2020
Contact name Jie Cao
Organization name Zhejiang University
Department Department of Polymer Science and Engineering
Street address Zheda Road 38
City Hangzhou
ZIP/Postal code 310027
Country China
Platforms (2)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (17)
GSM3769069 input mRNA
GSM3769070 a6A-IP mRNA
GSM3769071 cyclized a6A-IP mRNA
BioProject PRJNA543237
SRA SRP198610

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131316_HeLa.DEseq2.results.xlsx 1.9 Mb (ftp)(http) XLSX
GSE131316_HeLa_counts.txt.gz 5.1 Mb (ftp)(http) TXT
GSE131316_RAW.tar 234.6 Mb (http)(custom) TAR (of BED, TXT, XLS, XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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