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Series GSE131291 Query DataSets for GSE131291
Status Public on Jan 08, 2020
Title Gata3 directly regulates cell cycle exit-coupled lens fiber cell differentiation in mouse embryonic lens
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Gata3 is a DNA-binding transcription factor involved in cellular differentiation in a variety of tissues. To investigate the role of Gata3 in lens development we conducted lens-specific conditional loss-of-function model of Gata3 and confirmed abnormal regulation of cell cycle exit-coupled lens differentiation and retention of nuclei in the mutated lens fibers. We analyzed transcriptomes between control and mutated lenses by RNA-seq and revealed dysregulation of genes encoding critical components of the lens differentiation cascade, including a subset of crystallin genes, down-regulation of Dnase2b and reduced expression of Cdkn1b/p27 and Cdkn1c/p57.
Overall design Lens mRNA profiles of E14.5 wild-type (WT) and Gata3 lens-specific knockout (CKO) mice were analyzed by RNA-seq. Each sample represents the pool of 10 lenses from 5 embryos and total number of 3 biological replicates was used for transcriptome sequencing using Illumina HiSeq400 system.
Contributor(s) Martynova E, Zhao Y, Xie Q, Overbeek PA, Zheng D, Cvekl A
Citation(s) 31847788
Submission date May 15, 2019
Last update date Jan 08, 2020
Contact name Ales Cvekl
Organization name Albert Einstein College of Medicine
Department The Departments Ophthalmology and Visual Sciences, Genetics
Lab Dr. Cvekl's lab
Street address 1300 Morris Park Avenue, Ullman 123
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM3768818 WT1
GSM3768819 WT2
GSM3768820 WT3
BioProject PRJNA543127
SRA SRP198538

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Supplementary file Size Download File type/resource
GSE131291_Gata3_KO_VS_WTsummary_table_raw_counts_GEO.txt.gz 184.2 Kb (ftp)(http) TXT
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