|
| Status |
Public on Jan 08, 2020 |
| Title |
Gata3 directly regulates cell cycle exit-coupled lens fiber cell differentiation in mouse embryonic lens |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gata3 is a DNA-binding transcription factor involved in cellular differentiation in a variety of tissues. To investigate the role of Gata3 in lens development we conducted lens-specific conditional loss-of-function model of Gata3 and confirmed abnormal regulation of cell cycle exit-coupled lens differentiation and retention of nuclei in the mutated lens fibers. We analyzed transcriptomes between control and mutated lenses by RNA-seq and revealed dysregulation of genes encoding critical components of the lens differentiation cascade, including a subset of crystallin genes, down-regulation of Dnase2b and reduced expression of Cdkn1b/p27 and Cdkn1c/p57.
|
| |
|
| Overall design |
Lens mRNA profiles of E14.5 wild-type (WT) and Gata3 lens-specific knockout (CKO) mice were analyzed by RNA-seq. Each sample represents the pool of 10 lenses from 5 embryos and total number of 3 biological replicates was used for transcriptome sequencing using Illumina HiSeq400 system.
|
| |
|
| Contributor(s) |
Martynova E, Zhao Y, Xie Q, Overbeek PA, Zheng D, Cvekl A |
| Citation(s) |
31847788 |
| Submission date |
May 15, 2019 |
| Last update date |
Jan 08, 2020 |
| Contact name |
Ales Cvekl |
| E-mail(s) |
ales.cvekl@einstein.yu.edu
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
The Departments Ophthalmology and Visual Sciences, Genetics
|
| Lab |
Dr. Cvekl's lab
|
| Street address |
1300 Morris Park Avenue, Ullman 123
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA543127 |
| SRA |
SRP198538 |
Less...
More...