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Series GSE131272 Query DataSets for GSE131272
Status Public on Oct 04, 2019
Title Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In mice, spared nerve injury replicates symptoms of human neuropathic pain and
induces upregulation of many genes in somatosensory neurons. Here we used single cell transcriptomics to probe the effects of partial infraorbital transection of the trigeminal nerve at the cellular level. Uninjured neurons were unaffected by transection of major nerve branches, segregating into many different classes. In marked contrast, axotomy rapidly transformed damaged neurons into just two new and closely-related classes where almost all original identity was lost. Remarkably, sensory neurons also adopted this transcriptomic state following various minor peripheral injuries. By genetically marking injured neurons, we showed that the injuryinduced transformation was reversible, with damaged cells slowly reacquiring normal gene expression profiles. Thus, our data expose transcriptomic plasticity, previously thought of as a driver of chronic pain, as a programed response to many types of injury and a potential mechanism for regulating sensation during wound healing.
Overall design Nuclei from trigeminal ganglia from wildtype, spared nerve injury mice (2-, 7-, 21-, and 75 days after injury), chronic itch with facial lesions, and minor facial injury mice were isolated. Neuronal nuclei were purified with anti-NeuN antibody and single nucleus RNA-seq were performed with either the Drop-Seq or 10x Genomics method.
Contributor(s) Nguyen M, Ryba N
Citation(s) 31592768
Submission date May 15, 2019
Last update date Oct 11, 2019
Contact name Minh Q Nguyen
Organization name NIDCR/NIH
Street address 9000, Rockville Pike
City Bethesda
State/province MARYLAND
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (11)
GSM3768364 WT_TG_1_DropSeq
GSM3768365 WT_TG_2_DropSeq
GSM3768366 WT_TG_3_DropSeq
BioProject PRJNA543101
SRA SRP198498

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131272_D0-10x.csv.gz 9.3 Mb (ftp)(http) CSV
GSE131272_D0-Drop.csv.gz 5.1 Mb (ftp)(http) CSV
GSE131272_D2-10x.csv.gz 10.1 Mb (ftp)(http) CSV
GSE131272_D21-10x.csv.gz 8.8 Mb (ftp)(http) CSV
GSE131272_D7-10x.csv.gz 6.5 Mb (ftp)(http) CSV
GSE131272_D75-10x.csv.gz 9.3 Mb (ftp)(http) CSV
GSE131272_data-cuts.csv.gz 14.8 Mb (ftp)(http) CSV
GSE131272_data-lesions.csv.gz 13.6 Mb (ftp)(http) CSV
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Processed data are available on Series record

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