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Series GSE131139 Query DataSets for GSE131139
Status Public on Oct 28, 2020
Title H3K27ac and BRD4-binding regions in a clear cell renal cell carcinoma cell line, OS-RC-2
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Advanced clear cell renal cell carcinoma (ccRCC) frequently causes systemic inflammation. Here, we identified the functional role and regulatory mechanism of inflammation driven by advanced ccRCC cells. The inflammatory nature of advanced ccRCC was recapitulated in a preclinical model of ccRCC. Amplification of cancer cell-intrinsic inflammation during ccRCC progression triggered neutrophil-dependent lung metastasis. Massive expression of inflammation-related genes was transcriptionally activated by epigenetic remodelling through mechanisms such as DNA demethylation and super-enhancer formation. Bromodomain and extra-terminal motif inhibitor (BETi) synchronously suppressed C–X–C-type chemokines in ccRCC cells and decreased neutrophil-dependent lung metastasis. Overall, our findings shed insight into the nature of inflammatory ccRCC, which triggers metastatic cascades, and suggest a potential therapeutic strategy.
Overall design H3K27ac and BRD4 ChIP-seq analysis was performed with clear cell renal cell carcinoma cell lines, OS-RC-2 and its derivatives.
Contributor(s) Nishida J, Momoi Y, Tamura Y, Takahashi K, Koinuma D, Miyazono K, Ehata S
Citation(s) 32203421
Submission date May 13, 2019
Last update date Oct 28, 2020
Contact name Shogo Ehata
Organization name The University of Tokyo
Department Graduate School of Medicine
Lab Deaprtment of Molecular Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (16)
GSM3764594 H3K27ac binding region in OSPa, replicate 1
GSM3764595 H3K27ac binding region in OSPa, replicate 2
GSM3764596 H3K27ac binding region in OS5K #1, replicate 1
BioProject PRJNA542708
SRA SRP198302

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Supplementary file Size Download File type/resource
GSE131139_H3K27ac_ChIPseq_OS5K_1_SE.bed.gz 9.1 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OS5K_1_TE.bed.gz 487.9 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OS5K_2_SE.bed.gz 7.6 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OS5K_2_TE.bed.gz 501.9 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OS5K_3_SE.bed.gz 7.1 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OS5K_3_TE.bed.gz 523.4 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OSPa_SE.bed.gz 8.5 Kb (ftp)(http) BED
GSE131139_H3K27ac_ChIPseq_OSPa_TE.bed.gz 307.2 Kb (ftp)(http) BED
GSE131139_RAW.tar 767.9 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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