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Series GSE131086 Query DataSets for GSE131086
Status Public on Dec 15, 2020
Title Human Pluripotent Stem Cell-Derived Kidney Organoids for Modeling Epithelial Transport and Injury
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Maximizing the potential of human kidney organoids for drug testing, regenerative medicine and to model development and disease requires addressing cell immaturity, the lack of a branching collecting system and the off target cell types. Here we establish methods to independently generate the two kidney progenitor cell populations – metanephric mesenchyme and ureteric bud. Combining these two progenitor cell types results in organoids with an improved branched collecting system. We also identified the hormones aldosterone and arginine vasopressin as critical to promote maturation of collecting duct cell types. The resulting organoids contain the full range of epithelial cells in the nephron, including principal and intercalated cells. By scRNA-seq, we demonstrate superior proximal tubule maturation and reduced off-target cell populations using this protocol.
Overall design Single cell profiling of kidney organoids using 10x Chromium
Contributor(s) Humphreys BD
Citation(s) 33326782
Submission date May 13, 2019
Last update date Mar 16, 2021
Contact name Haojia Wu
Organization name Washington University in St. Louis
Department Renal Division/Internal Medicine
Lab Humphreys Lab
Street address 600 S Euclid Ave
City St. Louis
State/province Missouri
ZIP/Postal code MO 63110
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM3763146 Takasato organoid
GSM3763147 Uchimura organoid
BioProject PRJNA542638
SRA SRP198240

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131086_RAW.tar 71.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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