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Series GSE131007 Query DataSets for GSE131007
Status Public on May 09, 2020
Title Organ-specific tropism and adaptation after metastasis of ER+ breast cancers
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Patients diagnosed with estrogen receptor (ER) positive breast cancer have a prolonged risk of distal metastatic recurrence to vital organs. Metastatic disease is incurable at present due to the development of treatment resistant cell populations. Here we used single-cell RNA sequencing to evaluate the transcriptome heterogeneity of ER+ breast cancer patient-derived xenografts (PDX) tropic for three common breast cancer metastatic sites – bone, brain, and liver – compared to primary tumors grown in the mammary fat pad. Metastatic cell populations at each location were phenotypically distinct from primary tumor cells with unique transcriptional programs indicative of signaling programs driven by specific transcription factors. Cells that metastasized to brain and liver tissue adopted gene expression programs indicative of the target organ microenvironments. Discerning the organ-specific phenotypic adaptations of metastatic ER+ breast cancer cells may help tailor appropriate therapies for individual patients and to each metastatic site.
Overall design Single-cell RNA-seq analysis of human breast cancer patient derived xenografts in mice.
Contributor(s) Gillen AE, Padilla Just N, Finlay-Schultz J, Kriss MS, Riemondy KA, Costello JC, Buckner M, Wellberg EA, Cittelly DM, Burchill MA, Horwitz KB, Hesselberth JR, Kabos P, Sartorius CA
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Submission date May 10, 2019
Last update date May 09, 2020
Contact name Peter Kabos
Organization name University of Colorado School of Medicine
Department Division of Medical Oncology
Street address 12801 E. 17th Avenue
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (7)
GSM3759506 ucd46liver scRNA-seq
GSM3759507 ucd46tumor scRNA-seq
GSM3759508 ucd4liver scRNA-seq
BioProject PRJNA542275
SRA SRP197534

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Supplementary file Size Download File type/resource
GSE131007_ucd46_count_matrix.tsv.gz 3.4 Mb (ftp)(http) TSV
GSE131007_ucd4_count_matrix.tsv.gz 24.2 Mb (ftp)(http) TSV
GSE131007_ucd65_count_matrix.tsv.gz 5.6 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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