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Series GSE130919 Query DataSets for GSE130919
Status Public on Sep 13, 2019
Title Lgr5 and Col22a1 mark progenitor cells in the lineage toward juvenile articular chondrocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The synovial joint forms from a pool of progenitor cells in the interzone region of the future joint. Expression of Gdf5 and Wnt9a has been used to mark the earliest cellular processes in the formation of the interzone and the progenitor cells. However, progression and lineage specification toward the different tissues of the joint is not well understood. Here, by lineage tracing studies we identify a population of Lgr5+ interzone cells that contribute to the formation of cruciate ligaments, synovial membrane and articular chondrocytes of the joint. This is supported by single cell transcriptome analyses. Furthermore, we showed that Col22a1, a marker of early articular chondrocytes, co-expresses with Lgr5+ cells prior to cavitation as an important lineage marker specifying the progression towards articular chondrocytes. Lgr5+ cells contribute to the repair of a joint defect with the re-establishment of Col22a1 containing superficial layer.
Overall design Knee joint and surrounding tissue was obtained from E14.5 Lgr5GPF/+ ( embryos and digested with enzyme for 10x genomics single cell analysis
Contributor(s) Chan W, Lam Y, Chen P, Chan D
Citation(s) 31522976
Submission date May 08, 2019
Last update date Sep 19, 2019
Contact name Peikai Chen
Phone 852-258315217
Organization name The University of Hong Kong
Department Faculty of Medicine
Street address Sassoon Road 5
City Pok Fu Lam
ZIP/Postal code 10000
Country Hong Kong
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (1)
GSM3756179 Knee-joint at E14.5 single-cell RNAseq by 10X Genomics
BioProject PRJNA541943
SRA SRP197115

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Supplementary file Size Download File type/resource
GSE130919_RAW.tar 35.6 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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