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Series GSE130910 Query DataSets for GSE130910
Status Public on Jun 01, 2019
Title To investigate whether recover DNMT3A expression permitted adult beta cell of βRapKO establishing proper transcriptome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Murine islets were isolated from 8-week-old WT and βRapKO mice and initially recovered in 1640 RPMI supplemented with 10% serum, 11.1mM glucose overnight. Then islets were transfected with Lenti-GFP or Lenti-Dnmt3a for 24 hours, following cultured in 1640 RPMI medium for 48 hours.Islets were harvested and stored in -80°C for preparation for RNA sequencing.
Overall design Transcriptome of three groups: WT, βRapKO, βRapKO+LvDnmt3a
Contributor(s) Ni Q, Wang Q, Ning G
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Submission date May 08, 2019
Last update date Jun 01, 2019
Contact name Qicheng Ni
Phone +8615216616283
Organization name Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine
Street address 197 Ruijin 2nd Road
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3756058 WT rep1
GSM3756059 WT rep2
GSM3756060 KO rep1
BioProject PRJNA541937
SRA SRP197113

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Supplementary file Size Download File type/resource
GSE130910_gene_expression.txt.gz 656.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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