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Series GSE130880 Query DataSets for GSE130880
Status Public on Apr 28, 2020
Title Single cell RNA sequencing of murine liver CD45+ mononuclear cells in HBs-HepR mouse model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used immunocompetent Fah-/- mice as the recipients and adoptively transferred HBsAg+ hepatocytes from HBs-Tg mice to replace the recipient hepatocytes (HBs-HepR). HBs-HepR mice maintained persistent HBsAg expression with chronic hepatitis and spontaneous liver fibrosis, and eventually developed HCC with a prevalence of 100%.
 
Overall design HBs-HepR mice reconstruction for 0 week, 12 weeks and 18 weeks was harvested and liver MNCs were isolated and stained with anti-CD45 and 7-AAD. CD45+7-AAD− cells were sorted using the FACSAria II Cell Sorter. Single-cell barcoded scRNA-seq libraries using Chromium Single Cell 3′ Library V2 (10x Genomics) and sc-RNA-libraries were sequenced on HiSeq X Ten (Illumina).
 
Contributor(s) Hao X, Tian Z
Citation(s) 31767976
Submission date May 08, 2019
Last update date Apr 28, 2020
Contact name Xiaolei Hao
Organization name Institute of Immunology, USTC
Street address huangshan Road 443
City Hefei
State/province Anhui
ZIP/Postal code 230027
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (3)
GSM3755565 CD45+ mononuclear cells, 0w (10x scRNA)
GSM3755566 CD45+ mononuclear cells, 12w (10x scRNA)
GSM3755567 CD45+ mononuclear cells, 18w (10x scRNA)
This SubSeries is part of SuperSeries:
GSE130945 A novel mouse model of hepatocellular carcinoma mediated by hepatitis B virus-specific CD8+ T cells
Relations
BioProject PRJNA541902
SRA SRP197072

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Supplementary file Size Download File type/resource
GSE130880_RAW.tar 118.9 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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