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Series GSE130784 Query DataSets for GSE130784
Status Public on May 07, 2019
Title Ultra-High Throughput Single Cell Analysis of Proteins and RNAs by Split-pool Synthesis
Organisms Homo sapiens; Mus musculus
Experiment type Other
Expression profiling by high throughput sequencing
Summary We report a method enabling simultaneous, ultra-high throughput single-cell-barcoding, of millions of cells for targeted single cell analysis of proteins and RNAs. This method termed Quantum Barcoding (QBC) circumvents the need to isolate single cells by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (four 96-well plates and a multichannel pipette) cell-specific codes are added to each tagged molecule within cells. This is accomplished through sequential rounds of the well-established process of classic “split-pool synthesis”. We demonstrate the utility of this currently research use only technology in multiple model systems.
Overall design Analysis of various cell types by the QBC single-cell analysis technology
Contributor(s) O’Huallachain M, Paladugu S
Citation(s) 32382044
Submission date May 06, 2019
Last update date May 14, 2020
Contact name Maeve Ohuallachain
Street address 4300 Hacienda Drive
City Pleasanton
State/province CALIFORNIA
ZIP/Postal code 94588
Country USA
Platforms (6)
GPL15103 Illumina HiSeq 1000 (Mus musculus)
GPL15433 Illumina HiSeq 1000 (Homo sapiens)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (24)
GSM3753567 exp185T
GSM3753568 exp211-a1
GSM3753569 Exp180M
BioProject PRJNA541327
SRA SRP195639

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130784_RAW.tar 200.3 Mb (http)(custom) TAR (of TAR, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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