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Series GSE130768 Query DataSets for GSE130768
Status Public on May 06, 2020
Title Oncogene regulated release of extracellular vesicles
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Oncogenes reprogram multiple metabolic phenotypes of cancer cells including the balance between anabolic and catabolic processes, mechanisms of nutrient uptake, and choices in nutrient utilization. Here, we explore how different oncogenes regulate biomass loss via extracellular vesicle release. We use isogenic mammary breast epithelial cells transformed with a panel of oncogenes found commonly mutated, amplified or overexpressed in multiple cancers. We observe an increase in extracellular vesicle (EV) release upon oncogenic transformation, with MYC and AURKB oncogenes eliciting the highest number of EVs produced. Oncogene expression altered the protein composition of released EVs. Likewise, miRNAs were differentially sorted into EVs in an oncogene-specific manner. We performed an integrated pathway analysis of metabolites and gene expression across different oncogene-expressing cells and identified that ceramide-sphingosine metabolism was broadly deregulated, especially in MYC overexpressing cells. Inhibition of neutral sphingomyelinases (N-SMase) resulted in significant decrease in EV production in MYC high cells, while ESCRT-dependent small EV production predominated in AURKB cells.
Overall design Gene expression analysis using RNA-seq from three different oncogene-expressing (MYC, AURKB, HRas) and control (Puro/Blast) MCF10A cells to identify dysregulated metabolic pathways that are important for extracelluler vesicle biogenesis
Contributor(s) Goga A, Kilinc S
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Submission date May 06, 2019
Last update date May 06, 2020
Contact name Andrei Goga
Organization name UCSF
Department Cell & Tissue Biology
Lab Goga Lab
Street address 513 Parnassus Ave
City San Francisco
State/province CA
ZIP/Postal code 94143-0512
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM3753313 AurKB_1
GSM3753314 AurKB_2
GSM3753315 AurKB_3
BioProject PRJNA541302
SRA SRP195601

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Supplementary file Size Download File type/resource
GSE130768_gene.description.txt.gz 6.6 Mb (ftp)(http) TXT
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