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Series GSE130649 Query DataSets for GSE130649
Status Public on May 31, 2019
Title Defective Tmprss3-Associated Hair Cell Degeneration in Inner Ear Organoids
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The role of TMPRSS3 in hair cell (HC) remains unclear. Nelson and colleagues generated stem cell-derived inner ear organoids with Tmprss3-mutations to show that 1) inner ear organoids exhibit comparative effects of the genetic abnormality to its in vivo counterparts, 2) Tmprss3-mutations lead to apoptosis and reduced BK channels in HCs, and 3) cell membrane-bound TMPRSS3 is localized in HCs.
Overall design Single-cell RNA sequencing (scRNA-Seq) was performed with day 25 and day 35 organoids from R1/E (WT) and R1/E-Tmprss3KO-GFPKI (KO).
Contributor(s) Tang P, Nelson R
Citation(s) 31204303
Submission date May 02, 2019
Last update date Aug 30, 2019
Contact name Pei-Ciao Tang
Phone 3373260631
Organization name University of Miami
Department Otolayrngology
Street address 1501 NW 10th Avenue, Miami, FL 33136, 926 F2
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM3746278 R1E_D25-WT
GSM3746279 Tmprss3_D25-KO
GSM3746280 R1E_D35-WT
BioProject PRJNA540873
SRA SRP194649

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130649_RAW.tar 220.8 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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