|
Status |
Public on May 13, 2019 |
Title |
Mapping of microhomologies at CRISPR/Cas9 deletions |
Organism |
Mus musculus |
Experiment type |
Other
|
Summary |
The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterise unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger deletions are prevalent at multiple distinct loci on different chromosomes, in cultured cells and mouse embryos alike. We observed a high frequency of microhomologies at larger deletion breakpoint junctions, suggesting the involvement of microhomology-mediated end joining in their generation. In populations of edited cells, the distribution of larger deletion sizes is dependent on proximity to sgRNAs and cannot be predicted by microhomology sequences alone.
|
|
|
Overall design |
Targeted amplicon sequencing at two sites targeted using CRISPR/Cas9
|
|
|
Contributor(s) |
Owens DD, de Bruijn M |
Citation(s) |
31127293 |
|
Submission date |
May 02, 2019 |
Last update date |
Jun 07, 2019 |
Contact name |
Dominic Owens |
E-mail(s) |
dominic.owens@utoronto.ca
|
Organization name |
University of Oxford
|
Department |
MRC WIMM
|
Street address |
John Radcliffe Hospital
|
City |
Oxford |
ZIP/Postal code |
OX3 9DS |
Country |
United Kingdom |
|
|
Platforms (1) |
|
Samples (4)
|
GSM3744938 |
E14 mESCs CRISPR/Cas9 Site 7 replicate 1 |
GSM3744939 |
E14 mESCs CRISPR/Cas9 Site 7 replicate 2 |
GSM3744940 |
E14 mESCs CRISPR/Cas9 Site 9 replicate 1 |
GSM3744941 |
E14 mESCs CRISPR/Cas9 Site 9 replicate 2 |
|
Relations |
BioProject |
PRJNA540816 |
SRA |
SRP194544 |