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| Status |
Public on May 03, 2019 |
| Title |
Whole genome bisulfite genome analysis of OX40L-Jagged-1 expanded Treg and Teff cells |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
NOD mice were treated with PBS or OX40L and Jagged-1 proteins once a week for three weeks. A week after final treatment CD4+CD25- Teff cells and CD4+CD25hi Treg cells were sorted by FACS. Genomic DNA isolated from sorted cells and differences in DNA methylation pattern in these cell types between PBS and OX40L-Jagged-1 treated mice was analyzed by whole genome bi-sulfite sequencing.
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| |
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| Overall design |
Examination of methylation pattern in Teff vs Treg cells between PBS and OX40L-JAG1 mice.
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| |
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| Contributor(s) |
Kumar P, Maienschein-Cline M, Arbieva Z, Prabhakar B |
| Citation(s) |
31704879 |
| Submission date |
May 02, 2019 |
| Last update date |
Nov 13, 2019 |
| Contact name |
Prabhakaran Kumar |
| E-mail(s) |
praba.biopioneer@gmail.com
|
| Phone |
3122866678
|
| Organization name |
UIC
|
| Department |
Microbiology and Immunology
|
| Street address |
835 S Wolcott Ave.
|
| City |
Chiciago |
| State/province |
Illinois |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA540788 |
| SRA |
SRP194541 |
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