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Series GSE130560 Query DataSets for GSE130560
Status Public on Jul 20, 2021
Title Single-cell RNA-seq to decipher the subpopulations of human decidual leukocytes in normal and RSA pregnancies
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Maternal fetal immune tolerance is a biological event that plays a key role in pregnancy. In particular, the mechanism by which the maternal immune system does not exclude the semi-identical antigen of the fetus has not been fully answered so far. The most serious result of the maternal fetal immune tolerance balance is the recurrent spontaneous abortion (RSA). The fetus is rejected by the mother in the early pregnancy and cannot continue to develop in the uterus. Based on these scientific questions, we used single-cell sequencing technology to analyze nearly 30,000 leukocytes in the decidua of normal and RSA. A total of 13 immune cell subsets were found, of which dNK cells were more prominent, The lineage differentiation pathway of dNK cells was excavated, and the pathological role of dNK differentiation abnormality in the occurrence of RSA was analyzed. Our results deepen our understanding of the differentiation and function of human decidual leukocytes, especially dNK cells. We clearly recognize that the immune system has dynamic changes compared with normal pregnancy under pathological conditions, which is likely to occur in RSA. The important factors, we expect our results to provide a new basis for RSA's immune diagnosis and treatment strategy.
Overall design Single cell sequencing on decidual immune cellss was performed from normmal pregnancy women and recurrent spontaneous abortion (RSA) patients. CD45+ leukocytes were sorted from decidua at early pregnancy period through MoFLo XDP.
Contributor(s) Wang F, Wang Y
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Submission date May 01, 2019
Last update date Jul 20, 2021
Contact name Yan-Ling Wang
Phone 8601064807195
Organization name Institute of Zoology, Chinese Academy of Sciences
Department State Key Laboratory of Stem Cell and Reproductive Biology
Street address Beichen West Rd., Chaoyang, Beijing
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM3743501 ND_1 decidual leukocytes-scRNA-seq
GSM3743502 ND_2 decidual leukocytes-scRNA-seq
GSM3743503 ND_3 decidual leukocytes-scRNA-seq
BioProject PRJNA540611
SRA SRP194375

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Supplementary file Size Download File type/resource
GSE130560_matrix.RData.gz 73.5 Mb (ftp)(http) RDATA
GSE130560_phenotype.csv.gz 498.4 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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