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| Status |
Public on Feb 24, 2020 |
| Title |
Examining the role of ATRX-RNA interactions in ATRX and PRC2 localization and gene expression. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We disrupted the RNA binding region in endogenous ATRX and tested localization of ATRX, EZH2 and H3K27me3 by CUT&RUN. We show that ATRX-RNA binding is important to regulate ATRX and PRC2 localization to specific genes.
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| Overall design |
We performed CUT&RUN for ATRX, EZH2 and H3K27me3 in WT and ATRX△RBR (ATRX with RNA binding region deleted) using the protocol described in Skene et al, Nature protocols, 2018). RNA Seq was performed in WT and ATRX△RBR.
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| Contributor(s) |
Ren W, Sarma K |
| Citation(s) |
32376827 |
| |
| Submission date |
Apr 29, 2019 |
| Last update date |
May 18, 2020 |
| Contact name |
Kavitha Sarma |
| Organization name |
The Wistar Institute
|
| Street address |
3601 Spruce St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA540279 |
| SRA |
SRP194170 |
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