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| Status |
Public on Oct 11, 2019 |
| Title |
A revised model for promoter competition based on multi-way chromatin interactions at the α-globin locus |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Specific communication between gene promoters and enhancers is critical for accurate regulation of gene expression. However, it remains unclear how specific interactions between multiple regulatory elements contained within a single chromatin domain are coordinated. Recent technological advances which can detect multi-way chromatin interactions at single alleles can provide insights into how multiple regulatory elements cooperate or compete for transcriptional activation. Here, we use such an approach to investigate how interactions of the α-globin enhancers are distributed between multiple promoters in a mouse model in which the α-globin domain is extended to include several additional genes. Our data show that gene promoters do not form mutually exclusive interactions with enhancers, but all interact simultaneously in a single complex. These findings suggest that promoters do not structurally compete for interactions with enhancers, but form a regulatory hub structure, which is consistent with recent models of transcriptional activation occurring in non- membrane bound nuclear compartments.
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| Overall design |
Tri-C is a 3C-based technique that can analyze multi-way interactions. It combines 3C library preparation (NlaIII restriction enzyme) with oligonucleotide capture enrichment for viewpoints of interest and the fragments they interact with. The capture step has been optimized to capture two or more interacting fragments with high efficiency. We have used this to analyze the 3D structure of the active mouse alpha globin locus in primary erythroid cells.
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| Contributor(s) |
Oudelaar AM, Hughes JR |
| Citation(s) |
31776347 |
| |
| Submission date |
Apr 25, 2019 |
| Last update date |
Dec 02, 2019 |
| Contact name |
A Marieke Oudelaar |
| E-mail(s) |
marieke.oudelaar@imm.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
MRC Weatherall Institute of Molecular Medicine
|
| Street address |
John Radcliffe Hospital
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
|
| GSM3735878 |
Wild type mouse erythroid cells biological replicate 1 |
| GSM3735879 |
Wild type mouse erythroid cells biological replicate 2 |
| GSM3735880 |
Wild type mouse erythroid cells biological replicate 3 |
| GSM3735881 |
D3839 mouse erythroid cells biological replicate 1 |
| GSM3735882 |
D3839 mouse erythroid cells biological replicate 2 |
| GSM3735883 |
D3839 mouse erythroid cells biological replicate 3 |
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| Relations |
| BioProject |
PRJNA535476 |
| SRA |
SRP193847 |
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