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Series GSE130212 Query DataSets for GSE130212
Status Public on Apr 01, 2020
Title The single cell transcriptional landscape of female primordial germ cell differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Female primordial germ cell differentiation undergoes sex determination and meiosis initiation asynchronously. Here we investigate the transcriptional profiles of 20519 single cells collected from E12.5, E14.5 and E16.5 mouse fetal ovaries. Clustering analysis identifies ten clusters and defines dozens of corresponding marker genes and provides a global view of cellular differentiation from undifferentiated primordial germ cells towards meiotic oocytes. We explore the dynamics of gene expression within the differentiation trajectory with special focus on the mechanisms for meiotic initiation.
Overall design Single cell sequencing on primordial germ cells was performed. Oct4-EGFP+ primordial germ cells were sorted from E12.5, E14.5 and E16.5 fetal gonads through MoFLo XDP.
Contributor(s) Zhao Z, Ma J, Sun Q
Citation(s) 32716582
Submission date Apr 23, 2019
Last update date Jul 29, 2020
Contact name Qingyuan Sun
Phone 8601064807050
Organization name Institute of Zoology, Chinese Academy of Sciences
Department State Key Laboratory of Stem Cell and Reproductive Biology
Street address Beichen West Rd., Chaoyang
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (3)
GSM3733920 scRNA-seq-E12.5
GSM3733921 scRNA-seq-E14.5
GSM3733922 scRNA-seq-E16.5
BioProject PRJNA534295
SRA SRP193506

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Supplementary file Size Download File type/resource
GSE130212_PGC.txt.gz 329.6 Mb (ftp)(http) TXT
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