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| Status |
Public on Apr 01, 2020 |
| Title |
The single cell transcriptional landscape of female primordial germ cell differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Female primordial germ cell differentiation undergoes sex determination and meiosis initiation asynchronously. Here we investigate the transcriptional profiles of 20519 single cells collected from E12.5, E14.5 and E16.5 mouse fetal ovaries. Clustering analysis identifies ten clusters and defines dozens of corresponding marker genes and provides a global view of cellular differentiation from undifferentiated primordial germ cells towards meiotic oocytes. We explore the dynamics of gene expression within the differentiation trajectory with special focus on the mechanisms for meiotic initiation.
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| Overall design |
Single cell sequencing on primordial germ cells was performed. Oct4-EGFP+ primordial germ cells were sorted from E12.5, E14.5 and E16.5 fetal gonads through MoFLo XDP.
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| Contributor(s) |
Zhao Z, Ma J, Sun Q |
| Citation(s) |
32716582 |
| Submission date |
Apr 23, 2019 |
| Last update date |
Jul 29, 2020 |
| Contact name |
Qingyuan Sun |
| E-mail(s) |
sunqy@ioz.ac.cn
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| Phone |
8601064807050
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| Organization name |
Institute of Zoology, Chinese Academy of Sciences
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| Department |
State Key Laboratory of Stem Cell and Reproductive Biology
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| Street address |
Beichen West Rd., Chaoyang
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA534295 |
| SRA |
SRP193506 |
