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| Status |
Public on Apr 28, 2020 |
| Title |
Chromatin spreading in 3D through Insulator looping regulates genes through to H3K27me3 micro-domains |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We used the technology of ChIP-sequencing to analyze changes in H3K27me3 levels in Drosophila Schneider S2 depleted of endogenous Beaf32 and expressing insulator mutants ('mut-Beaf32' or 'muBeaf') as compared to control cells expressing wild-type Beaf32 (Control; 'CTL')
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| Overall design |
Drosophila Schneider S2 cells either containing endogenous Beaf32 or either transfected by mutated insulator looping mutants (mut-Beaf32 ) have been depleted of endogenous Beaf32. Quantification of H3K27me3 has been done using ChIP-seq on both depleted and wild-type condition.
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| Contributor(s) |
Heurteau A, Depierre D, Perrois C, Fosseprez O, Humbert J, Lhoumaud P, Schaak S, Cuvier O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 23, 2019 |
| Last update date |
Apr 28, 2020 |
| Contact name |
Olivier Cuvier |
| E-mail(s) |
olivier.cuvier@gmail.com
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| Organization name |
CNRS
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| Department |
LBME
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| Lab |
Chromatin Dynamics & Cell proliferation
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| Street address |
118 route de Narbonne
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| City |
Toulouse |
| ZIP/Postal code |
31062 |
| Country |
France |
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| Platforms (1) |
| GPL26560 |
BGISEQ-500 (Drosophila melanogaster) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA534289 |
| SRA |
SRP193502 |
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