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Status |
Public on Oct 02, 2019 |
Title |
Simultaneous measurement of DNA repair activities and mRNA abundance in single cells |
Organisms |
Homo sapiens; synthetic construct |
Experiment type |
Expression profiling by high throughput sequencing Other
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Summary |
New methods that measure the static abundance of DNA, RNA, and proteins in single cells are rapidly transforming biology. But these methods do not provide insight into dynamic cellular activities, precluding a complete understanding of cellular heterogeneity. We developed a method to measure enzymatic activities in single cells and used it to simultaneously measure DNA repair enzyme activities and mRNA expression. By including polyadenylated DNA hairpin substrates with defined lesions in a single-cell mRNA sequencing experiment, we measured repair activities by capturing repair intermediates and products catalyzed by activities in single cells.
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Overall design |
Single cell mRNA sequencing libraries and matching DNA repair activity libraries from the 10X genomics platform.
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Web link |
https://github.com/hesselberthlab/sc-haircut
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Contributor(s) |
Richer AL, Riemondy KA, Hesselberth JR |
Citation(s) |
32286626 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R35 GM119550 |
Coordination of RNA cleavage with end modification and processing |
UNIVERSITY OF COLORADO DENVER |
Jay R Hesselberth |
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Submission date |
Apr 22, 2019 |
Last update date |
Apr 18, 2020 |
Contact name |
Jay R. Hesselberth |
E-mail(s) |
jay.hesselberth@cuanschutz.edu
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Organization name |
University of Colorado School of Medicine
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Department |
Biochemistry and Molecular Gentetics
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Lab |
Jay Hesselberth
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Street address |
12801 E 17TH AVE
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City |
Aurora |
State/province |
CO |
ZIP/Postal code |
80045 |
Country |
USA |
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Platforms (2) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
GPL26526 |
Illumina NovaSeq 6000 (synthetic construct) |
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Samples (22)
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Relations |
BioProject |
PRJNA534019 |
SRA |
SRP193719 |