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Series GSE130117 Query DataSets for GSE130117
Status Public on Oct 02, 2019
Title Simultaneous measurement of DNA repair activities and mRNA abundance in single cells
Organisms Homo sapiens; synthetic construct
Experiment type Expression profiling by high throughput sequencing
Summary New methods that measure the static abundance of DNA, RNA, and proteins in single cells are rapidly transforming biology. But these methods do not provide insight into dynamic cellular activities, precluding a complete understanding of cellular heterogeneity. We developed a method to measure enzymatic activities in single cells and used it to simultaneously measure DNA repair enzyme activities and mRNA expression. By including polyadenylated DNA hairpin substrates with defined lesions in a single-cell mRNA sequencing experiment, we measured repair activities by capturing repair intermediates and products catalyzed by activities in single cells.
Overall design Single cell mRNA sequencing libraries and matching DNA repair activity libraries from the 10X genomics platform.
Web link
Contributor(s) Richer AL, Riemondy KA, Hesselberth JR
Citation(s) 32286626
NIH grant(s)
Grant ID Grant title Affiliation Name
R35 GM119550 Coordination of RNA cleavage with end modification and processing UNIVERSITY OF COLORADO DENVER Jay R Hesselberth
Submission date Apr 22, 2019
Last update date Apr 18, 2020
Contact name Jay R. Hesselberth
Organization name University of Colorado School of Medicine
Department Biochemistry and Molecular Gentetics
Lab Jay Hesselberth
Street address 12801 E 17TH AVE
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (2)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL26526 Illumina NovaSeq 6000 (synthetic construct)
Samples (22)
GSM3732360 barnyard_mrna
GSM3732361 barnyard_repair
GSM3732362 pbmc1_mrna
BioProject PRJNA534019
SRA SRP193719

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130117_RAW.tar 86.4 Mb (http)(custom) TAR (of MTX, TSV) 2.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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