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| Status |
Public on Jan 23, 2020 |
| Title |
Single cell transcriptome analysis of bleomycin injured distal adult murine lung airway epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To comprehensively study the heterogeneity within distal airway epithelium, we performed single cell transcriptomic analysis of the naive lung samples. Our analysis reveals that distal lung airway epithelial cells are highly heterogenous. We employed supervised clustering to identify a rare group of cells with transcriptional similiarities to embryonic distal lung bud tip progenitors. This approach identified a quiescent progenitor population of airway cells that accounted for all in vitro regenerative activity. Furthermore, these cells were also required for in vivo transplantation, reconstitution of alveoli, and functional recovery of injured mice. These findings indicate that small subpopulations of specialized stem/progenitors are required for effective lung regeneration and could potentially be used as therapeutic adjuncts after major lung injury.
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| Overall design |
We performed single cell transcriptomic sequencing of flow purified Epcam+/Itgb4+/CD200+ cells via 10X Genomics platform
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| Contributor(s) |
Kathiriya JJ, Chapman HA |
| Citation missing |
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| |
| Submission date |
Apr 19, 2019 |
| Last update date |
Jan 25, 2020 |
| Contact name |
Jaymin J Kathiriya |
| E-mail(s) |
jaymin.kathiriya@mssm.edu
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| Organization name |
University of California San Francisco
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| Department |
Medicine
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| Lab |
Chapman Lab
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| Street address |
513 Parnassus Ave
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94131 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA533796 |
| SRA |
SRP193093 |
