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Series GSE130077 Query DataSets for GSE130077
Status Public on Jan 23, 2020
Title Single cell transcriptome analysis of bleomycin injured distal adult murine lung airway epithelium
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To comprehensively study the heterogeneity within distal airway epithelium, we performed single cell transcriptomic analysis of the naive lung samples. Our analysis reveals that distal lung airway epithelial cells are highly heterogenous. We employed supervised clustering to identify a rare group of cells with transcriptional similiarities to embryonic distal lung bud tip progenitors. This approach identified a quiescent progenitor population of airway cells that accounted for all in vitro regenerative activity. Furthermore, these cells were also required for in vivo transplantation, reconstitution of alveoli, and functional recovery of injured mice. These findings indicate that small subpopulations of specialized stem/progenitors are required for effective lung regeneration and could potentially be used as therapeutic adjuncts after major lung injury.
Overall design We performed single cell transcriptomic sequencing of flow purified Epcam+/Itgb4+/CD200+ cells via 10X Genomics platform
Contributor(s) Kathiriya JJ, Chapman HA
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Submission date Apr 19, 2019
Last update date Jan 25, 2020
Contact name Jaymin J Kathiriya
Organization name University of California San Francisco
Department Medicine
Lab Chapman Lab
Street address 513 Parnassus Ave
City San Francisco
State/province CA
ZIP/Postal code 94131
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (1)
GSM3731533 B4CD200_SingleCell_Bleo
BioProject PRJNA533796
SRA SRP193093

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Supplementary file Size Download File type/resource
GSE130077_RAW.tar 103.5 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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