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Series GSE129896 Query DataSets for GSE129896
Status Public on Dec 30, 2019
Title Small RNA sequencing of mouse muscle and heart samples after small hairpin RNA delivery
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary RNA interference (RNAi) is a promising gene therapy strategy for targeting dominant mutations. This therapy leverages small hairpin RNAs (shRNAs) to knock down gene expression; however, delivery of too much shRNA can disrupt the processing of endogenous microRNAs (miRNAs), and lead to toxicity. In this study, we sought to understand the effect that excessive shRNAs have on muscle miRNAs by transducing several constructs in mice and performing small RNA sequencing on their muscle and liver tissues. We found that shRNA expression was highest in the heart, and that when shRNAs accumulated to about 27% of total small RNAs, mice experienced substantial cardiomyopathy. At this level in the heart, shRNAs in other muscle tissues were only ~1.8-7.6% of total miRNAs. Regardless of treatment, the predominant heart microRNAs remained stable across samples, while the lower-expressed miR-451 – one of the only microRNAs processed in a Dicer-independent manner – changed in direct correlation with shRNA level and toxicity. Our data suggest that certain muscle miRNAs compete with exogenous shRNAs, leading to the observed cardiomyopathy, and that the mechanism of cardiotoxicity in these mice in response shRNA treatment was in contrast to what has been previously shown in the liver. Quantifying microRNA profiles after excessive shRNA delivery illuminates the host response to rAAV-shRNA, allowing for safer and more robust therapeutic gene knockdown, and gives us insight into the basic functions of muscle microRNAs.
Overall design Small RNA sequencing of mouse liver and five muscle tissues (Diaphragm, Heart, Gastrocnemius, Quadriceps and Tibialis Anterior) in biological triplicate in three groups: uninjected mice, those with small hairpin RNAs (shRNAs) against the Beta-galactosidase gene with a 19nt stem or 21nt stem. Tissues were harvested 2 weeks, 6 weeks or 12 weeks after adeno-associated virus 6 delivery (AAV6-shRNA). All conditions had a minimum of 3 samples.
Contributor(s) Valdmanis PN, Course MM
Citation(s) 31927330
Submission date Apr 16, 2019
Last update date Jan 27, 2020
Contact name Paul Valdmanis
Phone 206-221-8059
Organization name University of Washington
Department Medicine
Lab Paul Valdmanis
Street address 1705 NE Pacific St, HSB J-309
City Seattle
State/province WA
ZIP/Postal code 98115-7720
Country USA
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (86)
GSM3724626 DIA_19nt_2w_repA
GSM3724627 DIA_19nt_2w_repB
GSM3724628 DIA_19nt_2w_repC
BioProject PRJNA533108
SRA SRP192739

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129896_muscle_miRNA_reads.txt.gz 57.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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