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Series GSE129883 Query DataSets for GSE129883
Status Public on Mar 05, 2020
Title RNA-seq of podocytes treated with glomerular endothelial cell vesicles
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The effect of miRNA delivery from glomerular endothelial cells (GEnCs) to podocytes in vitro was examined by mRNA epression in podocytes untreated and treated with extracellular vesicles from GEnCs under varied activation conditions.
 
Overall design RNA sequencing of human podocytes untreated (UT) or treated with extracellular vesicles (EVs) derived from human glomerular endothelial cells (GEnCs) for 24 hours. EVs were isolated by ultracentrifugation from supernatants of GEnCs in a steady state (SS) or activated with glucose (Glu) or puromycin aminonucleoside (PAN) for 24 hours.
 
Contributor(s) Hill N, Michell DL, Pusey C, Vickers KC, Woollard KJ
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Submission date Apr 16, 2019
Last update date Mar 05, 2020
Contact name Danielle L Michell
E-mail(s) danielle.michell@vumc.org
Organization name Vanderbilt Univ. Medical Center
Department Department of Medicine
Lab 312 Preston Research Bldg
Street address 2220 Pierece Ave
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM3724164 Untreated 1 [RNA-seq]
GSM3724165 Untreated 2 [RNA-seq]
GSM3724166 Untreated 3 [RNA-seq]
This SubSeries is part of SuperSeries:
GSE129892 Glomerular endothelial derived vesicles mediate podocyte dysfunction via extracellular transfer of miRNA-200c-3p
Relations
BioProject PRJNA533091
SRA SRP192716

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129883_Podocytes1086.count.vsd.txt.gz 1018.6 Kb (ftp)(http) TXT
GSE129883_Podocytes1086.fpkm.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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