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Series GSE129845 Query DataSets for GSE129845
Status Public on Aug 28, 2019
Title Single-cell transcriptomic map of the human and mouse bladders
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A comprehensive cellular anatomy of normal human bladder is vital to address the cellular origins of benign bladder disease and bladder cancer. The physiological function and pathological changes of bladder are associated with its cell type. To investigate the classification and underlying function of bladder cells, we conducted single-cell RNA sequencing (scRNA seq) of 12,423 cells from human bladder and 12,884 cells from mouse bladder. Here, we show a single-cell transcriptomic map of the human and mouse bladders, including 16 clusters of human bladder cells and 15 clusters of mouse bladder cells. Homology and heterogeneity of human and mouse were compared and analyzed by our study, and indicated that there were both conservative and heterogeneous aspects of human and mouse evolution. We also discovered two novel types of human bladder cells; one type is ADRA2A+ and HRH2+ interstitial cells which may be associated with nerve conduction and allergic reactions; the other type is TNNT1+ epithelial cells that may be different from other epithelial cells in biological function. Collectively, this analysis provides a resource for the discovery of novel cell type, specific transcription factors, signaling receptors, and genes dataset that will help us to study the relationship between cell types and diseases.
Overall design Primary bladder samples from 3 patients and C57BL/6 mice were used for single cell RNA sequencing by 10X Genomics
Contributor(s) Yu Z, Liao J, Chen Y, Zou C, Zhang H, Cheng J, Liu D, Li T, Zhang Q, Li J, Yang X, Ye Y, Huang Z, Long X, Yang R, Mo Z
Citation(s) 31462402
Submission date Apr 15, 2019
Last update date Sep 02, 2019
Contact name Zhenyuan Yu
Phone +8613617715617
Organization name Guangxi Medical University
Department Center for Genomic and Personalized Medicine
Street address Shuangyong road 22
City Nanning
State/province Guangxi
ZIP/Postal code 530021
Country China
Platforms (2)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (5)
GSM3723357 Patient 1 Bladder Homogenate
GSM3723358 Patient 2 Bladder Homogenate
GSM3723359 Patient 3 Bladder Homogenate
BioProject PRJNA532936
SRA SRP192646

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Supplementary file Size Download File type/resource
GSE129845_RAW.tar 178.7 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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