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Series GSE129724 Query DataSets for GSE129724
Status Public on Dec 31, 2019
Title Multi-step Epigenetic Programming of Monocytes during Infection
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Monocytes play a major role in the defense against pathogens. They are rapidly mobilized to inflamed sites where they exert both proinflammatory and regulatory effector functions. It is still poorly understood how this dynamic and exceptionally plastic system is controlled at the molecular level. Herein, we evaluated the differentiation process that occurs in Ly6Chi monocytes during oral infection by T. gondii. Flow cytometry and single-cell analysis revealed distinct activation status and gene expression profiles in the bone marrow, the spleen and the lamina propria of infected mice. We provide further evidence that this stepwise acquisition of effector functions, such as the capacity to produce interleukin-27, is accompanied by distinct waves of epigenetic programming, highlighting a role for STAT1/IRF1 in the bone marrow and AP-1/NF-kB in the periphery. This work broadens our understanding of the molecular events that occur in vivo during monocyte differentiation in response to inflammatory cues.
Overall design Study of chromatin accessibility in monocytes isolated from Bone Marrow, Spleen and Lamina Propria, in steady state or during oral Toxoplasma gondii infection.
Contributor(s) Detavernier A, Azouz A, Shehade H, Splittgerber M, Van Maele L, Nguyen M, Thomas S, Achouri Y, Svec D, Calonne E, Fuks F, Oldenhove G, Goriely S
Citation(s) 31872076
Submission date Apr 12, 2019
Last update date Dec 31, 2019
Contact name Stanislas Goriely
Phone +3226509586
Organization name Université Libre de Bruxelles
Street address Rue Adrienne Bolland 8
City Gosselies
ZIP/Postal code B-6041
Country Belgium
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (14)
GSM3720846 ATAC_MonoLow_Spleen_Toxo
GSM3720847 ATAC_MonoHigh_Spleen_Toxo
GSM3720848 ATAC_Mono_Spleen_SteadyState_rep1
BioProject PRJNA532592
SRA SRP192367

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Supplementary file Size Download File type/resource
GSE129724_RAW.tar 694.3 Mb (http)(custom) TAR (of BIGWIG, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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