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Series GSE129666 Query DataSets for GSE129666
Status Public on Apr 12, 2019
Title RNA sequence data in whole cell extracts of differentiated human podocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Sphingomyelin phosphodiesterase acid-like 3b (SMPDL3b) is a lipid raft enzyme that regulates plasma membrane (PM) fluidity. Here we report that SMPDL3b excess, as observed in podocytes in diabetic kidney disease (DKD), impairs insulin receptor isoform B-dependent pro-survival insulin signaling by interfering with insulin receptor isoforms binding to caveolin-1 in PM. SMPDL3b excess affects the production of active sphingolipids resulting in decreased ceramide-1-phosphate (C1P) content as observed in human podocytes in vitro and in kidney cortexes of diabetic db/db mice in vivo. Podocyte-specific Smpdl3b deficiency in db/db mice is sufficient to restore kidney cortex C1P content and to protect from DKD. Exogenous administration of C1P restores IR signaling in vitro and prevents established DKD progression in vivo. Taken together, we identified SMPDL3b as a modulator of insulin signaling and demonstrated that supplementation with exogenous C1P may represent a lipid therapeutic strategy to treat diabetic complications such as DKD.
Overall design RNA sequence data in whole cell extracts of differentiated human podocytes
RNA from Ctrl and SMPDL3b OE podocytes was isolated and analyzed for differences in the mRNA expression profile
RNA extraction, Preparation and sequencing RNA libraries, Illumina Sequencing RNA Data Analysis
Contributor(s) Mallela SK, Mitrofanova A
Citation(s) 31217420
Submission date Apr 11, 2019
Last update date Jul 01, 2019
Contact name Shamroop kumar Mallela
Organization name University of Miami
Department Medicine
Lab Nephrology
Street address 1580 NW 10th Ave
City Miami
State/province Florida
ZIP/Postal code 33136
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3733338 Podocytes_Ctrl 1
GSM3733339 Podocytes_Ctrl 2
GSM3733340 Podocytes_Ctrl 3
BioProject PRJNA532413
SRA SRP192089

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Supplementary file Size Download File type/resource
GSE129666_RNA_seq_data_SMP_to_Ctrl.xlsx 16.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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