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Series GSE129648 Query DataSets for GSE129648
Status Public on Jan 19, 2021
Title Regulation of Tfh differentiation by Mettl3 [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary T follicular helper (Tfh) cells are a specialized subset of CD4 T cells that are essential for most antibody productions and humoral responses. It is well characterized that transcriptional network exerts indispensable roles in Tfh cell differentiation. However, how post-transcriptional regulation controls Tfh biology has largely remained unclear. Multiple mice models were used here by a series of experimental approaches to illustrate m6A methyltransferase Mettl3 programs Tfh cell differentiation at post-transcriptional layer.
 
Overall design Ctrl and Mettl3-null Tfh cells (3 samples per group) or Th1 cells (2 samples per group) were sorted at day 8 after LCMV Armstrong infection. Then the total RNAs were extracted and subjected to RNA sequencing.
 
Contributor(s) Yao Y, Yu S
Citation(s) 33637761
Submission date Apr 11, 2019
Last update date Mar 10, 2021
Contact name Shuyang Yu
E-mail(s) ysy@cau.edu.cn
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (10)
GSM3718131 WT1
GSM3718132 WT2
GSM3718133 WT3
This SubSeries is part of SuperSeries:
GSE129650 m6A methyltransferase METTL3 regulates Tfh cell differentiation
Relations
BioProject PRJNA532393
SRA SRP192026

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129648_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
GSE129648_Tfh_rpkm.txt.gz 2.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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