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Series GSE129648 Query DataSets for GSE129648
Status Public on Jan 19, 2021
Title Regulation of Tfh differentiation by Mettl3 [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary T follicular helper (Tfh) cells are a specialized subset of CD4 T cells that are essential for most antibody productions and humoral responses. It is well characterized that transcriptional network exerts indispensable roles in Tfh cell differentiation. However, how post-transcriptional regulation controls Tfh biology has largely remained unclear. Multiple mice models were used here by a series of experimental approaches to illustrate m6A methyltransferase Mettl3 programs Tfh cell differentiation at post-transcriptional layer.
Overall design Ctrl and Mettl3-null Tfh cells (3 samples per group) or Th1 cells (2 samples per group) were sorted at day 8 after LCMV Armstrong infection. Then the total RNAs were extracted and subjected to RNA sequencing.
Contributor(s) Yao Y, Yu S
Citation(s) 33637761
Submission date Apr 11, 2019
Last update date Mar 10, 2021
Contact name Shuyang Yu
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (10)
GSM3718131 WT1
GSM3718132 WT2
GSM3718133 WT3
This SubSeries is part of SuperSeries:
GSE129650 m6A methyltransferase METTL3 regulates Tfh cell differentiation
BioProject PRJNA532393
SRA SRP192026

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129648_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
GSE129648_Tfh_rpkm.txt.gz 2.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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