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Series GSE129640 Query DataSets for GSE129640
Status Public on Apr 12, 2019
Title Multi-walled carbon nanotube-induced gene expression in vitro
Organism Homo sapiens
Experiment type Expression profiling by array
Summary There is a current interest in reducing the in vivo toxicity testing of nanomaterials in animals by increasing toxicity testing using in vitro cellular assays; however, toxicological results are seldom concordant between in vivo and in vitro models. This study compared global multi-walled carbon nanotube (MWCNT)-induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene expression from mouse lungs exposed to MWCNT. Using a cutoff of 10% false discovery rate and 1.5 fold change, we determined that there were more concordant genes (gene expression both up- or downregulated in vivo and in vitro) expressed in both cell types in coculture than in monoculture. When reduced to only those genes involved in inflammation and fibrosis, known outcomes of in vivo MWCNT exposure, there were more disease-related concordant genes expressed in coculture than monoculture. Additionally, different cellular signaling pathways are activated in response to MWCNT dependent upon culturing conditions. As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing.
 
Overall design SAEC and HMVEC coculture and mono-culture at 6 hr and 24 hr, correlated with in vivo animal studies
 
Contributor(s) Guo NL
Citation(s) 25511174, 31194188, 31146342
Submission date Apr 11, 2019
Last update date Jul 26, 2019
Contact name Nancy Lan Guo
E-mail(s) lguo@hsc.wvu.edu
Phone 3042936455
Organization name West Virginia University
Department Occupational and Environmental Health Sciences
Lab Mary Babb Randolph Cancer Center/Guo Laboratory
Street address 2816 Health Sciences Center
City Morgatown
State/province WV
ZIP/Postal code 26506-9300
Country USA
 
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (72)
GSM3717828 cocultured HMVEC DM control, biological replicate 1
GSM3717829 cocultured HMVEC DM control, biological replicate 2
GSM3717830 cocultured HMVEC DM control, biological replicate 3
Relations
BioProject PRJNA532386

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129640_RAW.tar 1.5 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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