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Series GSE129578 Query DataSets for GSE129578
Status Public on Apr 11, 2019
Title MULTI-seq: Universal sample multiplexing for single-cell RNA sequencing using lipid-tagged indices
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the development of MULTI-seq: a scRNA-seq and snRNA-seq sample multiplexing approach using lipid- or cholesterol-modified oligonucleotides. We demonstrate MULTI-seq utility in the following scRNA-seq and snRNA-seq contexts: (1) Live HEKs and HMECs with and without stimulation with TGF-β, (2) Purified nuclei from HEKs, MEFs, and Jurkats stimulated for 0-24 hrs with ionomycin and PMA, (3) 96 unique HMEC cultures (with one technical replicate), and (4) human metastases and mouse immune cells isolated from cryopreserved primary tissue samples dissected from a patient-derived xenograft mouse model of triple-negative breast cancer at progressive stages of metastasis to the lung.
Overall design Single-cell or single-nucleus gene expression profiles for (1) HEKs and HMECs, (2) HEKs, MEFs, and Jurkats, (3) HMECs in distinct culture conditions, and (4) Human metastases and mouse immune cells from PDX mice.
Contributor(s) McGinnis CS, Gartner ZJ
Citation(s) 31209384
Submission date Apr 10, 2019
Last update date Jun 26, 2019
Contact name Christopher S McGinnis
Organization name University of California, San Francisco
Department Pharmaceutical Chemistry
Lab Zev Gartner
Street address 600 16th Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (4)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL25431 Illumina HiSeq 4000 (Homo sapiens; Mus musculus)
Samples (24)
GSM3716609 POC_scRNAseq_LMO
GSM3716610 POC_scRNAseq_CMO
BioProject PRJNA531855
SRA SRP192397

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129578_HMEC1_techrep_matrix.mtx.gz 39.1 Mb (ftp)(http) MTX
GSE129578_HMEC2_techrep_matrix.mtx.gz 34.6 Mb (ftp)(http) MTX
GSE129578_HMEC3_techrep_matrix.mtx.gz 36.3 Mb (ftp)(http) MTX
GSE129578_HMEC4_techrep_matrix.mtx.gz 50.5 Mb (ftp)(http) MTX
GSE129578_HMEC_orig_matrix.mtx.gz 608.9 Mb (ftp)(http) MTX
GSE129578_PDX_hs_matrix.mtx.gz 5.9 Mb (ftp)(http) MTX
GSE129578_PDX_mm_matrix.mtx.gz 47.3 Mb (ftp)(http) MTX
GSE129578_POC_matrix.mtx.gz 269.3 Mb (ftp)(http) MTX
GSE129578_POC_nuc_matrix.mtx.gz 70.7 Mb (ftp)(http) MTX
GSE129578_processed_data_files.csv.tar.gz 3.7 Mb (ftp)(http) TAR
GSE129578_processed_data_files.tsv.tar.gz 32.4 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record

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