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| Status |
Public on Apr 30, 2019 |
| Title |
Gene Specific Activation Driven by Small Molecules |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Differing levels of chromatin compaction throughout the genome contribute to cell specificity and function. The importance of studying this complex processes is highlighted by the frequency of chromatin dysregulation in human diseases, which has led to the development of chromatin-based therapies in the clinic. To study this pathway, we designed a novel chemical-based system to redirect specific chromatin modifying machinery in a targeted and reversible manner. We synthesized a new class of bi-functional compounds, Chemical Epigenetic Modifiers (CEM)s, with one warhead arm that engages endogenous chromatin regulators, while the other targeting arm tethers the captured epigenetic regulatory machinery to the gene of interest. By redirecting endogenous chromatin regulatory machinery, we are able to control expression in a gene-specific manner.
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| Overall design |
Genome-wide profile for BRD4 in 293 HEK cells following a 48 hour treatment with CEM87, a bifunctional molecule that binds and redirects endogenous BRD4 to a specific locus via dCas9-CEM interaction.
RNA-seq in 293 HEK (4 samples)
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| Contributor(s) |
Gryder BE, Wen X, Khan J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 05, 2019 |
| Last update date |
Sep 22, 2019 |
| Contact name |
Javed Khan |
| E-mail(s) |
khanjav@mail.nih.gov
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| Phone |
2407606135
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| Organization name |
NCI, NIH
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| Department |
Genetics Branch
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| Lab |
Javed Khan
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| Street address |
37 Convent Dr.
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA531181 |
| SRA |
SRP191117 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE129407_RAW.tar |
1.3 Mb |
(http)(custom) |
TAR (of BED, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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