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Series GSE129351 Query DataSets for GSE129351
Status Public on Apr 02, 2020
Title Octameric nucleosomes are present within the inner kinetochore of human centromeres
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The centromere is a vital locus on each chromosome which seeds the kinetochore, allowing for a physical connection between the chromosome and the mitotic spindle. At the heart of the centromere is the centromere-specific histone H3 variant CENP-A/CENH3. Throughout the cell cycle the constitutive centromere associated network is bound to CENP-A chromatin, but how this protein network modifies CENP-A nucleosome dynamics in vivo is unknown. Here, using a combination of biophysical and biochemical analyses we provide evidence for the existence of two populations of structurally distinct CENP-A nucleosomes that co-exist at human centromeres. These two populations display unique sedimentation patterns in a glycerol gradient, and CENP-A nucleosomes that are physically associated with the inner kinetochore appear stabilized in an octameric conformation, with restricted access to the nucleosomal DNA by DNase I. In contrast, the bulk population of CENP-A nucleosomes have diminished heights and weakened DNA interactions. These data suggest that, in vivo, a reserve pool of immature CENP-A nucleosomes exist which wrap DNA loosely, whereas an active inner kinetochore complex associates with stabilized CENP-A nucleosomes. Our data have implications for a function for CENP-A that may be independent of its role in mitotis.
Overall design ChIP-seq
Contributor(s) Melters DP, Rakshit T, Grigoryev SA, Sturgill D, Dalal Y
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Submission date Apr 04, 2019
Last update date Apr 03, 2020
Contact name David Sturgill
Phone 240-760-6725
Organization name NIH
Department NCI
Street address Building 41, Rm B622
City Bethesda
State/province MARYLAND
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21311 PacBio RS II (Homo sapiens)
Samples (4)
GSM3711473 HeLa_ACA_IP
GSM3711474 HeLa_CENP-C_IP
GSM3711475 HeLa_Mock_IP
BioProject PRJNA531000
SRA SRP190889

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129351_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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