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Series GSE12922 Query DataSets for GSE12922
Status Public on Nov 07, 2008
Title Two Supporting Factors Greatly Improve the Efficiency of Human iPS Cell Generation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human fibroblasts can be induced into pluripotent stem cells (iPS cells), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4 and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPS cell generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations. We further demonstrated that by using a novel combination of the four factors OCT4, SOX2, KLF4 and UTF1, iPS cells could be generated at a frequency at least 10 times higher than using the original four reprogramming factors without c-MYC. The iPS cells generated in this work have a similar gene expression profile and differentiation potential as human embryonic stem (hES) cells. In conclusion, two novel supporting factors that increase the efficiency of direct reprogramming have been identified, and a more-efficient method for the generation of human iPS cells has been developed in the absence of the oncogene c-MYC.

Keywords: cell type comparison
Overall design Total RNA from hFSF, hAFF, hES cells (H1, H7) and 7 established iPS cell lines were labeled with Cy5, hybridized to a human Oligo Microarray (Phalanx Human Whole Genome OneArrayâ„¢, Phalanx Biotech) according to the manufacturer's protocol. Three technical repetitions were performed. After hybridization, arrays were scanned using GenePix 4000B scanner (Molecular Devices) and processed using the GenePix Pro 6.0 software (Molecular Devices). After removing control probes, a 14/33 presence call (SNR>=5 and foreground-background>0) was used to filter probes for the 33 microarrays, resulting in 12311 probes for further quantile normalization.
Contributor(s) Zhao Y, Yin X, Qin H, Deng H
Citation(s) 18983962
Submission date Sep 24, 2008
Last update date Aug 28, 2014
Contact name hongkui deng
Phone 86-10-6275-6474
Fax 86-10-6275-6474
Organization name Peking University
Department College of Life Sciences
Street address Yiheyuan Road 5#, Haidian District
City Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL6254 Phalanx Human OneArray
Samples (33)
GSM324142 H1_rep1
GSM324143 H1_rep2
GSM324144 H1_rep3
BioProject PRJNA110837

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Supplementary file Size Download File type/resource
GSE12922_Cluster.tif 2.8 Mb (ftp)(http) TIFF
GSE12922_RAW.tar 97.8 Mb (http)(custom) TAR (of GPR)
GSE12922_Replicates_Median_for_cluster.xls 1.9 Mb (ftp)(http) XLS
Processed data included within Sample table

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