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| Status |
Public on Apr 03, 2019 |
| Title |
Cognate T cell interactions affect the DC transcriptome [dataset 4] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our data suggest that peptide-presenting DC receive signals from synapse-forming antigen specific CD4+ or CD8+ T cells that alter the DC expression profile. To further test this notion, we hock immunized T cell-engrafted mice with NP harboring CpG and coated with OVA (OVA / CpG NP) or the control antigen BSA (BSA / CpG NP) and isolated NP+ DC for RNAseq.
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| Overall design |
NP+ DC were isolated 20 hrs post sub-cutaneous immunization with (OVA/CpG) NP or (BSA/CpG) NP from popliteal and inguinal lymph nodes. (OVA / CpG) or (BSA/CpG) NP+ DC were single-cell sorted into 384w-plates, barcoded and MARS-sequenced.
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| Contributor(s) |
Curato C, Jaitin DA, Giladi A, David E, Leshkowitz D, Jung S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 02, 2019 |
| Last update date |
Apr 04, 2019 |
| Contact name |
Steffen Jung |
| E-mail(s) |
s.jung@weizmann.ac.il
|
| Phone |
0097289342787
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| Organization name |
The Weizmann Institute of Science
|
| Department |
Immunology and Regenerative Biology
|
| Lab |
Steffen Jung
|
| Street address |
Herzl
|
| City |
REHOVOT |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE129215 |
IL-23 producing IL-10Rα-deficient gut macrophages elicit an IL-22-driven pro-inflammatory epithelial cell response |
|
| Relations |
| BioProject |
PRJNA530541 |
| SRA |
SRP190166 |
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